Xu Min-Dan, Wu Xian-Zheng, Zhou Yun, Xue Ying, Zhang Ke-Qin
Department of Endocrinology and Metabolism, Shanghai Tongji Hospital, Tongji University School of Medicine Shanghai, China.
Department of Emergency, Shanghai Tongji Hospital, Tongji University School of Medicine Shanghai, China.
Am J Transl Res. 2016 Jan 15;8(1):209-20. eCollection 2016.
This study aimed to evaluate the proteomic characteristics of plasma microparticles (MPs) from patients with newly diagnosed type 2 diabetes (T2DM).
The subjects comprised eight male T2DM patients recruited between December 2013 and March 2014, as well as eight age and sex-matched healthy controls enrolled during the same period. Plasma microparticles (MPs) were extracted from the blood of each subject, and subjected to proteomics analysis using label-free methods. Bioinformatic analyses were performed using specialized software.
3,148 unique peptides and 496 proteins were identified, among these, 46 proteins were differentially expressed between the two groups. Among these 46 candidates, 20 proteins had higher expression in T2DM group compared with the control group, whereas 3 proteins displayed lower expression. There were 17 proteins only detected in T2DM group, and 6 proteins only detected in the control group. Gene ontology (GO) analysis revealed significant differences between the two groups in some functional nodes, including neutrophil accumulation, chemokine production, platelet activation, and blood coagulation. Pathway analysis showed that proteins involved in platelet activation, cell adhesion, focal adhesion, and extracellular matrix-receptor interaction were differentially expressed between the 2 groups. Network analysis indicated that ubiquitin was the protein with the highest degree of connectivity.
Blood MPs from T2DM patients are enriched in proteins involved in platelet activation, cell adhesion, and inflammation. Therefore, MPs in T2DM patients might be associated with hypercoagulable state in diabetic patients and the development of diabetic complications.
本研究旨在评估新诊断的2型糖尿病（T2DM）患者血浆微颗粒（MPs）的蛋白质组学特征。
研究对象包括2013年12月至2014年3月招募的8名男性T2DM患者，以及同期纳入的8名年龄和性别匹配的健康对照。从每个受试者的血液中提取血浆微颗粒（MPs），并使用无标记方法进行蛋白质组学分析。使用专门软件进行生物信息学分析。
共鉴定出3148种独特肽段和496种蛋白质，其中46种蛋白质在两组之间存在差异表达。在这46种候选蛋白中，与对照组相比，20种蛋白在T2DM组中表达较高，而3种蛋白表达较低。有17种蛋白仅在T2DM组中检测到，6种蛋白仅在对照组中检测到。基因本体（GO）分析显示两组在一些功能节点上存在显著差异，包括中性粒细胞聚集、趋化因子产生、血小板活化和血液凝固。通路分析表明，参与血小板活化、细胞粘附、粘着斑和细胞外基质-受体相互作用的蛋白质在两组之间存在差异表达。网络分析表明泛素是连接度最高的蛋白质。
T2DM患者的血液MPs富含参与血小板活化、细胞粘附和炎症的蛋白质。因此，T2DM患者的MPs可能与糖尿病患者的高凝状态及糖尿病并发症的发生有关。
