Department of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Thromb Res. 2012 Aug;130(2):253-8. doi: 10.1016/j.thromres.2011.10.014. Epub 2011 Nov 17.
Circulating tissue factor positive microparticles (MPTF) were reported in a wide range of diseases with thrombotic tendency. Though D-dimer assay had a high negative predictive value for deep venous thrombosis (DVT) recurrence, there are currently no reliable positive predictors for recurrent DVT. We therefore quantified MPTF in patients with acute recurrent DVT to determine whether MPTF levels could be used to predict recurrent DVT.
Microparticles (MPs) were isolated from plasma of initial DVT patients (n=25), recurrent DVT patients (n=25) and sex- and age-matched healthy individuals (n=25), stained with annexin V, cell-specific monoclonal antibodies (MoAbs) and a MoAb directed against tissue factor (TF), and analyzed by flow cytometry. We also determined the plasma procoagulant activity with a Human TF Chromogenic Activity Assay Kit.
We found total MPTF to be elevated in recurrent DVT patients versus normal individuals (P=0.001). The number of monocyte-derived MPTF in both initial and recurrent DVT was higher than in normal individuals (P<0.01, respectively). The platelet and endothelial cell derived MPTF in recurrent DVT were significantly increased relative to other MPTF (P<0.05), although there was no difference between initial DVT patients and normal individuals. We demonstrated elevated procoagulant activity of platelet-free plasma in DVT patients relative to normal individuals, and a positive correlation with MPTF.
The elevated MPTF could be a potentially predictor for DVT recurrence. Further studies are needed to validate its sensitivity and specificity.
循环组织因子阳性微粒（MPTF）在多种具有血栓倾向的疾病中均有报道。尽管 D-二聚体检测对深静脉血栓（DVT）复发具有很高的阴性预测值，但目前尚无可靠的阳性预测因子用于预测复发性 DVT。因此，我们对急性复发性 DVT 患者进行了 MPTF 定量检测，以确定 MPTF 水平是否可用于预测复发性 DVT。
从初发 DVT 患者（n=25）、复发性 DVT 患者（n=25）和性别及年龄匹配的健康个体（n=25）的血浆中分离出微粒（MPs），用 Annexin V、细胞特异性单克隆抗体（MoAb）和针对组织因子（TF）的 MoAb 进行染色，并用流式细胞术进行分析。我们还使用 Human TF 显色活性检测试剂盒测定了血浆促凝血活性。
我们发现复发性 DVT 患者的总 MPTF 高于正常个体（P=0.001）。初发和复发性 DVT 患者的单核细胞衍生 MPTF 数量均高于正常个体（P<0.01）。复发性 DVT 患者的血小板和内皮细胞衍生 MPTF 明显高于其他 MPTF（P<0.05），而初发 DVT 患者与正常个体之间无差异。我们证明了 DVT 患者无血小板血浆的促凝血活性相对于正常个体升高，且与 MPTF 呈正相关。
升高的 MPTF 可能是 DVT 复发的潜在预测因子。需要进一步的研究来验证其敏感性和特异性。
