影响鸡胚肝细胞组织型聚集的特定因素。

Sankaran L, Proffitt R T, Petersen J R, Pogell B M

Proc Natl Acad Sci U S A. 1977 Oct;74(10):4486-90. doi: 10.1073/pnas.74.10.4486.

Conditions are described for the reproducible assay of substances affecting the in vitro rate of aggregation of isolated chick embryo hepatocytes. Two low molecular weight (less than 1000) fractions--one that promotes hepatocyte aggregation (HAP) and the other that inhibits this stimulation (HAI)--have been isolated and partially purified from adult chicken liver. One major active component of HAP was identified as taurine (2-aminoethanesulfonate). The presence of HAP during the entire time of assay was required for largest aggregate formation. HAP had no effect on aggregation of chick embryo neural retina, kidney, or heart cells. Our results and the fact that puromycin completely inhibits aggregate formation suggest that HAP and HAI influence the specific synthesis and interaction of membrane macromolecules involved in the aggregation process.

描述了用于可重复测定影响离体鸡胚肝细胞体外聚集速率的物质的条件。已从成年鸡肝脏中分离并部分纯化了两种低分子量（小于1000）的组分——一种促进肝细胞聚集（HAP），另一种抑制这种刺激（HAI）。HAP的一种主要活性成分被鉴定为牛磺酸（2-氨基乙磺酸盐）。为了形成最大的聚集体，在整个测定过程中都需要HAP的存在。HAP对鸡胚神经视网膜、肾脏或心脏细胞的聚集没有影响。我们的结果以及嘌呤霉素完全抑制聚集体形成这一事实表明，HAP和HAI影响参与聚集过程的膜大分子的特定合成和相互作用。