Balsamo J, Lilien J
Proc Natl Acad Sci U S A. 1974 Mar;71(3):727-31. doi: 10.1073/pnas.71.3.727.
A new assay for tissue-type-specific adhesive macromolecules is described. This assay measures the binding to whole cells of radioactive macromolecules in aggregation-enhancing supernatants. We show for two tissue types, embryonic chick neural retina and cerebrum, that binding is tissue-type specific. Specific binding can be distinguished from nonspecific binding by its sensitivity to displacement with horse serum and by the kinetics of binding. Specific binding appears to be a cooperative phenomenon.
本文描述了一种针对组织类型特异性黏附大分子的新检测方法。该检测方法测量聚集增强上清液中放射性大分子与全细胞的结合情况。我们针对两种组织类型,即胚胎期鸡的神经视网膜和大脑,证明了这种结合具有组织类型特异性。通过其对马血清置换的敏感性以及结合动力学,可以将特异性结合与非特异性结合区分开来。特异性结合似乎是一种协同现象。