Al-Shaaibi Siham N K, Waly Mostafa I, Al-Subhi Lyutha, Tageldin Mohamed H, Al-Balushi Nada M, Rahman Mohammad S
Department of Food Science and Nutrition, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat 123, Sultanate of Oman.
Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat 123, Sultanate of Oman.
Prev Nutr Food Sci. 2016 Mar;21(1):14-23. doi: 10.3746/pnf.2016.21.1.14. Epub 2016 Mar 31.
Non-alcoholic fatty liver disease (NAFLD) is caused by fat accumulation and is associated with oxidative stress. In this study, we investigated the potential protective effect of pomegranate (Punica granatum L.) peel extract (PPE) against oxidative stress in the liver of rats with NAFLD. Sprague-Dawley rats were fed a high fat diet (HFD), 20% corn oil, or palm oil for 8 weeks in the presence or absence of PPE. The control group was fed a basal diet. The progression of NAFLD was evaluated histologically and by measuring liver enzymes (alanine transaminase and aspartate transaminase), serum lipids (triglycerides and total cholesterol), and oxidative stress markers. The HFD feeding increased the body weight and caused NAFLD, liver steatosis, hyperlipidemia, oxidative stress, and elevated liver enzymes. Administration of PPE ameliorated the hepatic morphology, reduced body weight, improved liver enzymes, and inhibited lipogenesis. Furthermore, PPE enhanced the cellular redox status in the liver tissue of rats with NAFLD. Our findings suggest that PPE could improve HFD-induced NAFLD via abolishment of hepatic oxidative damage and hyperlipidemia. PPE might be considered as a potential lead material in the treatment of NAFLD and obesity through the modulation of lipid metabolism.
非酒精性脂肪性肝病（NAFLD）是由脂肪堆积引起的，与氧化应激相关。在本研究中，我们调查了石榴（Punica granatum L.）果皮提取物（PPE）对NAFLD大鼠肝脏氧化应激的潜在保护作用。将斯普拉格-道利大鼠在有或无PPE的情况下，喂食高脂肪饮食（HFD）、20%玉米油或棕榈油8周。对照组喂食基础饮食。通过组织学检查以及测量肝酶（丙氨酸转氨酶和天冬氨酸转氨酶）、血脂（甘油三酯和总胆固醇）和氧化应激标志物来评估NAFLD的进展。喂食HFD增加了体重并导致NAFLD、肝脏脂肪变性、高脂血症、氧化应激和肝酶升高。给予PPE改善了肝脏形态，减轻了体重，改善了肝酶，并抑制了脂肪生成。此外，PPE增强了NAFLD大鼠肝脏组织中的细胞氧化还原状态。我们的研究结果表明，PPE可以通过消除肝脏氧化损伤和高脂血症来改善HFD诱导的NAFLD。通过调节脂质代谢，PPE可能被视为治疗NAFLD和肥胖症的潜在先导物质。
