Kim Mina, Kim Dae Keun, Cha Youn-Soo
1 Department of Food Science and Human Nutrition, and Research Institute of Human Ecology, Chonbuk National University , Jeonju, Korea.
2 College of Pharmacy, Woosuk University , Jeonju, Korea.
J Med Food. 2016 May;19(5):442-9. doi: 10.1089/jmf.2015.3598. Epub 2016 Apr 12.
Adzuki beans have long been cultivated as a food and folk medicine in East Asia. In this study, we investigated the effect of black adzuki bean (BAB) extract on pancreatic cells and determined their mechanism of action in impaired glucose tolerance in an animal model of type 2 diabetes. In addition, we performed functional gene annotation analysis to identify genes related to the regulation of glucose metabolism and insulin response. Treatment of pancreatic β cells with BAB extract (0.2 mg/mL) led to tolerance of the high glucose-induced glucotoxicity, resulting in a similar viability as cells maintained in normal glucose media. In addition, dietary supplementation with BAB extract significantly (P < .05) improved hyperglycemia and homeostasis model assessment of insulin resistance index (HOMA-IR) in high-fat diet-induced glucose-intolerant obese C57BL/6J mice. Our results suggest that BAB extract ameliorates hyperglycemia and glucose intolerance, and lowers HOMA-IR by regulating insulin secretion and response, and by maintaining the integrity of pancreatic β cells exposed to hyperglycemic conditions.
赤小豆在东亚长期以来一直被作为食物和民间药物种植。在本研究中，我们研究了黑赤小豆（BAB）提取物对胰腺细胞的作用，并在2型糖尿病动物模型中确定了其在糖耐量受损中的作用机制。此外，我们进行了功能基因注释分析，以鉴定与葡萄糖代谢调节和胰岛素反应相关的基因。用BAB提取物（0.2mg/mL）处理胰腺β细胞可导致对高葡萄糖诱导的糖毒性的耐受性，从而使其活力与维持在正常葡萄糖培养基中的细胞相似。此外，在高脂饮食诱导的糖耐量不耐受肥胖C57BL/6J小鼠中，饮食补充BAB提取物显著（P<0.05）改善了高血糖和胰岛素抵抗指数的稳态模型评估（HOMA-IR）。我们的结果表明，BAB提取物通过调节胰岛素分泌和反应，以及维持暴露于高血糖条件下的胰腺β细胞的完整性，改善高血糖和糖耐量不耐受，并降低HOMA-IR。
