Yu Jiang-yong, Chen Qiu-fang, Lu Guo-yong
Zhongguo Zhong Yao Za Zhi. 2015 Nov;40(21):4200-4.
To establish a new method for simultaneously determining the content of five gingerol compounds in different processing degrees of ginger charcoal and PCA principal component analysis was conducted for analysis. Samples were analyzed on Ultimate TM XB-C18 column (4.6 mm x 250 mm, 5 μm) , with acetonitrile (A) -0.1% phosphoric acid solution (B) as mobile phase for gradient elution. Detection wavelength was set at 280 nm. The flow rate was 0.6 mL x min(-1) and the column temperature was 30 degrees C. The five compounds were separated well and showed good linearity (r ≥ 0.999 7) within the concentration ranges tested. The average value for recoveries was between 98.86% - 101.5% (RSD 1.4% - 2.9%). The contents of five compounds showed difference among different processing degrees of ginger charcoal. Zingiberone had the highest content in the standard carbon, and the content of gingerol was decreased as the deepening of processing degree. Different processing degrees of ginger charcoal were classified into three groups with PCA, and provided scientific basis for establishing the quality standards of ginger charcoal.
建立一种同时测定不同炮制品干姜中5种姜辣素类成分含量的新方法，并采用PCA主成分分析法进行分析。采用Ultimate TM XB-C18柱（4.6 mm×250 mm，5μm）分析样品，以乙腈（A）-0.1%磷酸溶液（B）为流动相进行梯度洗脱。检测波长设定为280 nm。流速为0.6 mL·min⁻¹，柱温为30℃。5种化合物分离良好，在所测浓度范围内线性关系良好（r≥0.999 7）。平均回收率在98.86%～101.5%之间（RSD为1.4%～2.9%）。5种化合物的含量在不同炮制品干姜中存在差异。姜酮在炮姜中含量最高，姜辣素含量随炮制程度加深而降低。采用PCA将不同炮制品干姜分为3类，为干姜质量标准的制定提供了科学依据。
