Liu Mei, Xia Xinhua, Chou Guixin, Liu Dong, Zuberi Aamir, Ye Jianping, Liu Zhijun
J AOAC Int. 2014 Jan-Feb;97(1):50-7. doi: 10.5740/jaoacint.12-437.
In the present study, an HPLC-DAD method was optimized for the quantitative determination of 6-gingerol, 6-shogaol, 8-gingerol, and 10-gingerol in ginger extracts. A chromatographic fingerprinting method was also established to differentiate and evaluate the ginger extracts for bioactivity. Twenty-one extracts were prepared by methods differing in ginger type (fresh versus dried), solvent, and extraction methods. The ANOVA analysis showed the methods' influence on the mean extraction yields of gingerols increased in the order of: high pressure-high temperature (HP)>blender (BD)>low pressure (LP). The optimal solvent to extract gingerols was found to be 95% ethanol. The type of ginger used had significant effects on the content of gingerols, but its overall influence depended on the solvent used. In order to maximize the extraction efficiency of gingerols, a combination of dry ginger, 95% ethanol, and the HP extraction method should be employed. The chromatographic fingerprints were obtained to differentiate the unknown components from all ginger extracts. The similarity of the chromatographic fingerprints was used to evaluate the differences among all extracts. It can be concluded that the chromatographic fingerprints are able to ensure the stability of each extract and have some correlation with the observed bioactivity.
在本研究中，优化了一种HPLC-DAD方法，用于定量测定生姜提取物中的6-姜酚、6-姜烯酚、8-姜酚和10-姜酚。还建立了一种色谱指纹图谱方法，以区分和评估生姜提取物的生物活性。通过不同的生姜类型（新鲜与干燥）、溶剂和提取方法制备了21种提取物。方差分析表明，这些方法对姜酚平均提取率的影响按以下顺序增加：高压-高温（HP）>搅拌器（BD）>低压（LP）。发现提取姜酚的最佳溶剂是95%乙醇。所用生姜的类型对姜酚含量有显著影响，但其总体影响取决于所用溶剂。为了使姜酚的提取效率最大化，应采用干姜、95%乙醇和HP提取方法的组合。获得了色谱指纹图谱以区分所有生姜提取物中的未知成分。色谱指纹图谱的相似度用于评估所有提取物之间的差异。可以得出结论，色谱指纹图谱能够确保每种提取物的稳定性，并且与观察到的生物活性有一定相关性。
