Hu Wen-Jing, Yu Ai-Qi, Bi Hai-Zheng, Zhang Zhao-Jiong, Wang Zhi-Bin, Wang Meng, Kuang Hai-Xue
Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin, China.
Front Mol Biosci. 2025 Mar 4;12:1530469. doi: 10.3389/fmolb.2025.1530469. eCollection 2025.
INTRODUCTION: Ginger, as a traditional Chinese medicine (TCM), can be used in clinical practice to treat various diseases. The product of ginger processed at high temperatures is called carbonized ginger (CG), which has a hemostatic effect that ginger originally did not have. The purpose of this study is to investigate the hemostatic effect of CG and the substances that exert hemostatic effects. METHODS: CG was prepared and successfully obtained CG carbon dots (CG-CDs) from its aqueous solution. After fully characterizing its structural information, the hemostatic effect was evaluated using mouse tail bleeding and liver injury bleeding models, and the clotting time was evaluated using capillary coagulation experiments. In addition, the hemostatic mechanism of CG-CDs was explored. RESULTS: The average particle size of CG-CDs was observed to be 4.07 nm and the lattice spacing was 0.216 nm. It was mainly composed of graphite structured carbon, with the main constituent elements being C, N, and O, containing functional groups such as C=N, C=O, and C-OH. The FL spectrum showed that the maximum excitation wavelength of CG-CDs was 360 nm, and the maximum emission wavelength was 470 nm. The QY of CG-CDs was calculated to be 0.45%. CG-CDs shortened bleeding time, reduced bleeding volume, and also shortened the time for blood clotting. With the increase of CG-CDs, the values of FIB gradually increased, and the PT values gradually decreased. In addition, CG-CDs increased PLT count, increased PLT activating factor TXB2, decreased 6-keto-PGF , increased PAI-1, and decreased t-PA. CONCLUSION: CG-CDs obtained from CG has hemostatic activity, mainly by activating exogenous coagulation and co-coagulation pathways, increasing PLT count, increasing PLT activating factor TXB2, reducing 6-keto-PGF , increasing PAI-1, and reducing t-PA, thereby affecting the fibrinolytic system and other pathways to exert hemostatic effects.
引言:生姜作为一种传统中药,可用于临床治疗多种疾病。经高温炮制的生姜制品称为炮姜(CG),其具有生姜原本没有的止血作用。本研究旨在探究炮姜的止血作用及其发挥止血作用的物质。 方法:制备炮姜并从其水溶液中成功获得炮姜碳点(CG-CDs)。在充分表征其结构信息后,使用小鼠尾出血和肝损伤出血模型评估止血效果,并通过毛细管凝血实验评估凝血时间。此外,还探究了CG-CDs的止血机制。 结果:观察到CG-CDs的平均粒径为4.07nm,晶格间距为0.216nm。它主要由石墨结构的碳组成,主要组成元素为C、N和O,含有C=N、C=O和C-OH等官能团。荧光光谱表明,CG-CDs的最大激发波长为360nm,最大发射波长为470nm。计算得出CG-CDs的量子产率为0.45%。CG-CDs缩短了出血时间,减少了出血量,还缩短了血液凝固时间。随着CG-CDs浓度的增加,纤维蛋白原(FIB)值逐渐升高,凝血酶原时间(PT)值逐渐降低。此外,CG-CDs增加了血小板(PLT)计数,增加了血小板激活因子血栓素B2(TXB2),降低了6-酮-前列腺素F1α(6-keto-PGF),增加了纤溶酶原激活物抑制剂-1(PAI-1),降低了组织型纤溶酶原激活剂(t-PA)。 结论:从炮姜中获得的CG-CDs具有止血活性,主要通过激活外源性凝血和内源性凝血途径,增加PLT计数,增加PLT激活因子TXB2,降低6-keto-PGF,增加PAI-1,降低t-PA,从而影响纤溶系统等途径发挥止血作用。
Front Mol Biosci. 2025-3-4
Nanoscale Adv. 2024-9-25
Colloids Surf B Biointerfaces. 2025-7
Nanomedicine (Lond). 2018-1-17
Front Pharmacol. 2023-8-10
J Chromatogr B Analyt Technol Biomed Life Sci. 2016-2-1
Spectrochim Acta A Mol Biomol Spectrosc. 2023-10-5
Int J Nanomedicine. 2024
Int J Biol Macromol. 2024-12
Eur J Med Chem. 2024-12-15
Int J Biol Macromol. 2024-11