Manabe T, Yamamoto H, Okuyama T
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
Electrophoresis. 1989 Mar;10(3):172-7. doi: 10.1002/elps.1150100303.
An apparatus for fully automated capillary isotachophoresis was constructed. A commercial apparatus (Shimadzu IP-2A) was modified in the electrolyte pumping system and the flow lines were simplified. An automatic sampler was used for sequential sampling. The equipment, pumps, the sampler, a high-voltage DC power supply, and a recorder, were controlled by a system controller which comprises a microcomputer and a BASIC program for time-control of the equipments. The apparatus was successfully used for the automated sequential analysis of human serum proteins. Forty serum samples were analyzed within 17 h without manual operation and for each sample the serum proteins were resolved into about twenty UV peaks or shoulders.
构建了一种全自动毛细管等速电泳装置。对一台商用仪器（岛津IP - 2A）的电解质泵送系统进行了改造，并简化了流路。使用自动进样器进行连续进样。该设备、泵、进样器、高压直流电源和记录仪由一个系统控制器控制，该系统控制器包括一台微型计算机和一个用于设备时间控制的BASIC程序。该装置成功用于人血清蛋白的自动连续分析。在无需人工操作的情况下，17小时内分析了40个血清样本，每个样本中的血清蛋白被分离为约20个紫外峰或峰肩。
