Böttcher A, Möllers C, Lackner K J, Schmitz G
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany.
Electrophoresis. 1998 Jun;19(7):1110-6. doi: 10.1002/elps.1150190710.
An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200-fold higher sample load compared to separation in capillaries of 180 microm inner diameter as used in analytical capillary isotachophoresis (C-ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C-ITP, which is essential for preparative-scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C-ITP and agarose gel electrophoresis.
描述了一种用于生物聚合物制备分离的自动自由溶液等速电泳系统（FS-ITP），其以批处理模式运行。与分析型毛细管等速电泳（C-ITP）中使用的内径为180微米的毛细管分离相比，分离室的尺寸允许样品负载量提高多达1200倍。该系统的制备能力在毫克范围内。该方法与分析型C-ITP完全兼容，这对于制备规模的等速电泳在优化电解质系统和寻找合适的间隔物方面至关重要。作为一个模型应用，报道了人血清蛋白的分离。通过C-ITP和琼脂糖凝胶电泳对收集的馏分进行分析。
