Games P D, Alves S N, Katz B B, Tomich J M, Serrão J E
Department of General Biology, State University of Viçosa, Viçosa, Brazil.
Department of Biology, State University of São João del-Rey, Divinópolis, Brazil.
Med Vet Entomol. 2016 Sep;30(3):253-63. doi: 10.1111/mve.12172. Epub 2016 Apr 13.
Mosquitoes are vectors for pathogens of malaria, lymphatic filariasis, dengue, chikungunya, yellow fever and Japanese encephalitis. Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) is a known vector of lymphatic filariasis. Its control in Brazil has been managed using the organophosphate temephos. Studies examining the proteins of Cx. quinquefasciatus that are differentially expressed in response to temephos further understanding of the modes of action of the insecticide and may potentially identify resistance factors in the mosquito. In the present study, a comparative proteomic analysis, using 2-dimensional electrophoresis coupled with matrix-assisted laser desorption/ionization (MALDI) time of flight (TOF)/TOF mass spectrometry, and bioinformatics analyses were performed to identify midgut proteins in Cx. quinquefasciatus larvae that were differentially expressed in response to exposure to temephos relative to those in untreated controls. A total of 91 protein spots were differentially expressed; 40 were upregulated and 51 were downregulated by temephos. A total of 22 proteins, predominantly upregulated, were identified as known to play a role in the immune response, whereas the downregulated proteins were involved in energy and protein catabolism. This is the first proteome study of the midgut of Cx. quinquefasciatus and it provides insights into the molecular mechanisms of insecticide-induced responses in the mosquito.
蚊子是疟疾、淋巴丝虫病、登革热、基孔肯雅热、黄热病和日本脑炎病原体的传播媒介。致倦库蚊（Culex quinquefasciatus Say，1823年）（双翅目：蚊科）是已知的淋巴丝虫病传播媒介。在巴西，对其控制一直使用有机磷杀虫剂双硫磷。研究致倦库蚊中因双硫磷而差异表达的蛋白质，有助于进一步了解该杀虫剂的作用模式，并可能识别蚊子中的抗性因素。在本研究中，采用二维电泳结合基质辅助激光解吸/电离（MALDI）飞行时间（TOF）/TOF质谱进行比较蛋白质组分析，并进行生物信息学分析，以鉴定致倦库蚊幼虫中，相对于未处理对照，因接触双硫磷而差异表达的中肠蛋白质。总共91个蛋白质斑点差异表达；40个上调，51个下调。总共鉴定出22种蛋白质，主要是上调的，已知在免疫反应中起作用，而下调的蛋白质则参与能量和蛋白质分解代谢。这是首次对致倦库蚊中肠进行蛋白质组研究，为了解蚊子中杀虫剂诱导反应的分子机制提供了见解。
