Monteiro Hugo R, Pestana João L T, Soares Amadeu M V M, Devreese Bart, Lemos Marco F L
Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
MARE-Marine and Environmental Sciences Centre, ESTM, Polytechnic of Leiria, 2520-641 Peniche, Portugal.
Toxics. 2020 Dec 11;8(4):117. doi: 10.3390/toxics8040117.
The potential of proteome responses as early-warning indicators of insecticide exposure was evaluated using the non-biting midge (Meigen) as the model organism. larvae were exposed to environmentally relevant concentrations of the neurotoxic pesticide spinosad to uncover molecular events that may provide insights on the long-term individual and population level consequences. The iTRAQ labeling method was performed to quantify protein abundance changes between exposed and non-exposed organisms. Data analysis revealed a general dose-dependent decrease in the abundance of globin proteins as a result of spinosad exposure. Additionally, the downregulation of actin and a larval cuticle protein was also observed after spinosad exposure, which may be related to previously determined life-history traits impairment and biochemical responses. Present results suggest that protein profile changes can be used as early warning biomarkers of pesticide exposure and may provide a better mechanistic interpretation of the toxic response of organisms, aiding in the assessment of the ecological effects of environmental contamination. This work also contributes to the understanding of the sublethal effects of insecticides in invertebrates and their molecular targets.
以摇蚊（Meigen）为模式生物，评估了蛋白质组反应作为杀虫剂暴露早期预警指标的潜力。将幼虫暴露于环境相关浓度的神经毒性杀虫剂多杀菌素中，以揭示可能为长期个体和种群水平后果提供见解的分子事件。采用iTRAQ标记方法量化暴露和未暴露生物体之间蛋白质丰度的变化。数据分析显示，由于多杀菌素暴露，珠蛋白的丰度普遍呈剂量依赖性下降。此外，多杀菌素暴露后还观察到肌动蛋白和一种幼虫表皮蛋白的下调，这可能与先前确定的生活史特征受损和生化反应有关。目前的结果表明，蛋白质谱变化可作为农药暴露的早期预警生物标志物，并可能为生物体的毒性反应提供更好的机制解释，有助于评估环境污染的生态影响。这项工作也有助于理解杀虫剂对无脊椎动物的亚致死效应及其分子靶点。
