Qiu Zhan-Dong, Deng Gang, Yang Jia, Min Zhe, Li Da-Yong, Fang Yu, Zhang Su-Ming
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
J Huazhong Univ Sci Technolog Med Sci. 2016 Apr;36(2):174-180. doi: 10.1007/s11596-016-1562-2. Epub 2016 Apr 13.
The present study aimed to improve the processing of data acquired from laser speckle contrast imaging (LSCI) to provide a standardization method to explore changes in regional cerebral blood flow (rCBF) and to determine the correlations among rCBF, cerebral ischemic lesion volume and microvascular density over time in a focal ischemic region. C57BL/6J mice were subjected to focal photothrombotic (PT) ischemia. rCBF was measured using LSCI at different time points before and after PT ischemia through an intact skull. Standardized rCBF (SrCBF), defined as the ratio of rCBF measured in the ipsilateral region of interest (ROI) to that in the corresponding contralateral region, was calculated to evaluate potential changes. In addition, the volume of the ischemic lesion and the microvascular density were determined using Nissl staining and immunofluorescence, respectively. The relationships among the ischemic lesion volume, microvascular density and SrCBF were analyzed over time. The results showed that the cortical rCBF measured using LSCI following PT ischemia in the C57BL/6J mice gradually increased. Changes in the cerebral ischemic lesion volume were negatively correlated with SrCBF in the ischemic region. Changes in the microvascular density were similar to those observed in SrCBF. Our findings indicate that LSCI is a practical technique for observing changes in murine cortical rCBF without skull opening and for analyzing the relationships among the ischemic lesion volume, microvascular density and SrCBF following focal cerebral ischemia. Preliminary results also suggest that the use of LSCI to observe the formation of collateral circulation is feasible.
本研究旨在改进从激光散斑对比成像（LSCI）获取的数据处理方法，以提供一种标准化方法来探究局部脑血流量（rCBF）的变化，并确定局灶性缺血区域随时间推移rCBF、脑缺血病变体积和微血管密度之间的相关性。将C57BL/6J小鼠进行局灶性光血栓形成（PT）缺血处理。通过完整颅骨在PT缺血前后的不同时间点使用LSCI测量rCBF。计算标准化rCBF（SrCBF），其定义为在同侧感兴趣区域（ROI）测量的rCBF与相应对侧区域测量的rCBF之比，以评估潜在变化。此外，分别使用尼氏染色和免疫荧光法测定缺血病变体积和微血管密度。分析缺血病变体积、微血管密度和SrCBF之间随时间的关系。结果表明，C57BL/6J小鼠PT缺血后使用LSCI测量的皮质rCBF逐渐增加。脑缺血病变体积的变化与缺血区域的SrCBF呈负相关。微血管密度的变化与SrCBF的变化相似。我们的研究结果表明，LSCI是一种实用技术，可用于在不打开颅骨的情况下观察小鼠皮质rCBF的变化，并分析局灶性脑缺血后缺血病变体积、微血管密度和SrCBF之间的关系。初步结果还表明，使用LSCI观察侧支循环的形成是可行的。
