Chai Cheng-Zhi, Mo Wei-Lan, Zhuang Xian-Fei, Kou Jun-Ping, Yan Yong-Qing, Yu Bo-Yang
Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.
Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.
Evid Based Complement Alternat Med. 2016;2016:4682786. doi: 10.1155/2016/4682786. Epub 2016 Mar 17.
Right ventricular (RV) dysfunction and failure contribute to the increasing morbidity and mortality of cardiovascular diseases; however, current treatment strategies are grossly inadequate. Sheng-Mai-San (SMS) has been used to treat heart diseases for hundreds of years in China, and its protective effects on RV have not been observed. The present study was to investigate the protective effects of SMS aqueous extract on RV dysfunction in chronic intermittent hypoxia (CIH) mice model. The results showed that CIH mice model presented RV dysfunction and maladaptive compensation after 28-day-CIH and SMS treatment significantly reversed these changes. Diastolic function of RV was restored and systolic dysfunction was attenuated, including elevation of RV stroke volume and fractional shortening, as well as pulmonary circulation. Structurally, SMS treatment inhibited RV dilation, cardiomyocytes vacuolization, ultrastructure abnormalities, and the expression of cleaved caspase-3. Of importance, SMS showed remarkable antioxidant activity by decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), increasing the levels of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1), as well as inhibiting the overexpression of 3-NT in RV. Our results indicate that SMS preserve RV structure and function in CIH-exposed mice by involving regulation in both ROS and Reactive Nitrogen Species (RNS) production.
右心室（RV）功能障碍和衰竭导致心血管疾病的发病率和死亡率不断上升；然而，目前的治疗策略严重不足。生脉散（SMS）在中国已被用于治疗心脏病数百年，但其对右心室的保护作用尚未见报道。本研究旨在探讨生脉散水提取物对慢性间歇性缺氧（CIH）小鼠模型右心室功能障碍的保护作用。结果显示，CIH小鼠模型在28天CIH后出现右心室功能障碍和适应性不良代偿，而SMS治疗显著逆转了这些变化。右心室舒张功能得以恢复，收缩功能障碍得到减轻，包括右心室每搏输出量和缩短分数的升高，以及肺循环的改善。在结构上，SMS治疗抑制了右心室扩张、心肌细胞空泡化、超微结构异常以及裂解的半胱天冬酶-3的表达。重要的是，SMS通过降低丙二醛（MDA）和4-羟基壬烯醛（4-HNE）水平、提高超氧化物歧化酶（SOD）和血红素加氧酶-1（HO-1）水平以及抑制右心室中3-硝基酪氨酸（3-NT）的过表达，显示出显著的抗氧化活性。我们的结果表明，生脉散通过调节活性氧（ROS）和活性氮（RNS）的产生，保护CIH暴露小鼠的右心室结构和功能。
