Department of Complex Prescription of TCM, China Pharmaceutical University , Nanjing , P R China.
Pharm Biol. 2013 Oct;51(10):1219-27. doi: 10.3109/13880209.2013.784920. Epub 2013 Jun 14.
Sheng-Mai-San (SMS) has been used for the treatment of cardiovascular disease for many years in China.
This study investigated the protective effects and active ingredients of SMS on myocardial injury (MI) in mice.
SMS and n-butanol extraction of SMS (SMS-Bu) were prepared and administered to ISO-treated mice once a day for 7 consecutive days. The doses were equivalent to the raw medicinal herbs of SMS 5.72, 2.86 and 1.43 g/kg/d, respectively. Propranolol was used as positive control. Serum biomarkers, histopathological and electrocardiographic were evaluated.
Serum lactate dehydrogenase, creatine kinase and myeloperoxidase increased to 4473.6 ± 322.5, 950.0 ± 35.0 and 90.4 ± 12.2 U/L in the model group. SMS and SMS-Bu groups showed a decrease from 10 to 29% for lactate dehydrogenase and from 17 to 42% for creatine kinase, respectively. Both SMS and SMS-Bu significantly attenuated the myeloperoxidase activities (from 42 to 56%) and malondialdehyde levels (from 25 to 45%) compared with the model group. Decreased superoxide dismutase activities in ISO-treated mice were elevated from 19 to 59% when treated with SMS and SMS-Bu. These biochemical results were supported by electrocardiogram (ECG) and histopathological observations. Furthermore, 8 ginsenosides and 16 lignans were identified in SMS-Bu.
These findings suggested that SMS-Bu was the mainly active fraction of SMS which exerted its beneficial effects on MI mainly through protecting myocardial tissue and reducing oxidative damage, and the ginsenosides and lignans may serve as active ingredients of SMS for the treatment of MI.
生脉散在中国已被用于治疗心血管疾病多年。
本研究旨在探讨生脉散（SMS）及其正丁醇提取物（SMS-Bu）对小鼠心肌损伤（MI）的保护作用及活性成分。
制备生脉散及其正丁醇提取物（SMS-Bu），并在 ISO 处理的小鼠中连续 7 天每天给药一次。剂量分别相当于生药生脉散 5.72、2.86 和 1.43 g/kg/d，普萘洛尔作为阳性对照。评估血清生物标志物、组织病理学和心电图。
模型组血清乳酸脱氢酶、肌酸激酶和髓过氧化物酶分别升高至 4473.6±322.5、950.0±35.0 和 90.4±12.2 U/L。SMS 和 SMS-Bu 组乳酸脱氢酶降低 10%29%，肌酸激酶降低 17%42%。与模型组相比，SMS 和 SMS-Bu 均能显著降低髓过氧化物酶活性（降低 42%56%）和丙二醛水平（降低 25%45%）。ISO 处理的小鼠中超氧化物歧化酶活性降低，用 SMS 和 SMS-Bu 处理后可升高 19%~59%。这些生化结果得到心电图（ECG）和组织病理学观察的支持。此外，在 SMS-Bu 中鉴定出 8 种人参皂苷和 16 种木脂素。
这些发现表明，SMS-Bu 是 SMS 的主要活性部位，其通过保护心肌组织和减少氧化损伤对 MI 发挥有益作用，人参皂苷和木脂素可能是 SMS 治疗 MI 的活性成分。
