Lin Cheng, Zhao Yuanli, Wan Gang, Zhu Anlin, Wang Hao
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China; Department of Neurosurgery, Civil Aviation General Hospital, Beijing 100123, P.R. China.
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.
Exp Ther Med. 2016 Apr;11(4):1355-1360. doi: 10.3892/etm.2016.3082. Epub 2016 Feb 16.
The aim of the current study was to observe the effects of simvastatin and taurine on delayed cerebral vasospasm (DCVS) following experimental subarachnoid hemorrhage (SAH) in rabbits. A total of 48 New Zealand white rabbits were allocated at random into four groups (control, SAH, SAH + simvastatin and SAH + taurine groups; n=12 each). The rabbit model of DCVS was established using a double hemorrhage method, which involved injecting autologous arterial blood into the cisterna magna in the SAH groups. The SAH + simvastatin group was administered oral simvastatin (5 mg/kg) daily between days 0-6. The SAH + taurine group was administered oral taurine (50 mg/kg) daily between days 0-6. Starch (50 mg/kg) was administered orally to the animals in the other two groups (control and SAH groups). The control group were not subjected to any other injections or treatment. The internal diameter and internal diameter/wall thickness of the basilar artery (BA) were measured. The expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were determined using immunohistochemical and quantitative polymerase chain reaction methods following the sacrifice of all animals on day 7. The activity of nuclear factor (NF)-κB in the BA was also measured using an electrophoretic mobility shift assay. The BA walls in the SAH + simvastatin and SAH + taurine groups exhibited reduced narrowing and corrugation of the tunica elastica interna compared with the SAH group. At the protein and cDNA levels, it was found that cerebral vasospasm of the BA in the SAH + simvastatin and SAH + taurine groups was alleviated, as indicated by the reduced expression of TNF-α, IL-1β, IL-6 and NF-κB compared with the SAH group (P<0.05). In conclusion, simvastatin and taurine reduced DCVS following SAH in rabbits, which suggests that these compounds may exert anti-inflammatory effects.
本研究的目的是观察辛伐他汀和牛磺酸对兔实验性蛛网膜下腔出血（SAH）后迟发性脑血管痉挛（DCVS）的影响。将48只新西兰白兔随机分为四组（对照组、SAH组、SAH + 辛伐他汀组和SAH + 牛磺酸组；每组n = 12）。采用双次出血法建立DCVS兔模型，SAH组通过向枕大池注入自体动脉血来建立模型。SAH + 辛伐他汀组在第0至6天每天口服辛伐他汀（5 mg/kg）。SAH + 牛磺酸组在第0至6天每天口服牛磺酸（50 mg/kg）。另外两组（对照组和SAH组）的动物口服淀粉（50 mg/kg）。对照组未接受任何其他注射或治疗。测量基底动脉（BA）的内径和内径/壁厚。在第7天处死所有动物后，采用免疫组织化学和定量聚合酶链反应方法测定肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和IL-6的表达水平。还采用电泳迁移率变动分析测定BA中核因子（NF）-κB的活性。与SAH组相比，SAH + 辛伐他汀组和SAH + 牛磺酸组的BA壁内弹性膜狭窄和皱缩减轻。在蛋白质和cDNA水平上，发现SAH + 辛伐他汀组和SAH + 牛磺酸组BA的脑血管痉挛得到缓解，与SAH组相比，TNF-α、IL-1β、IL-6和NF-κB的表达降低（P<0.05）。总之，辛伐他汀和牛磺酸可减轻兔SAH后的DCVS，这表明这些化合物可能发挥抗炎作用。
