Wang Siyang, Shao Xin, Li Xiaoxue, Su Xiaojuan, Huo Yongxu, Yang Chunlei
Department of Life Sciences, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Mol Clin Oncol. 2016 Apr;4(4):579-583. doi: 10.3892/mco.2016.764. Epub 2016 Feb 3.
Hypoxia-inducible factor-1 (HIF-1α) exerts an important role in protecting against cardiac tissue damage, for example, following ischemia-reperfusion (I/R), although the time frame during which it acts has yet to be fully elucidated. In the present study, a culture model of myocardial cells from Sprague-Dawley rats was used to examine the expression levels of HIF-1α and various downstream effectors at different times following I/R. The levels of HIF-1α were manipulated by overexpressing HIF-1α prior to I/R. HIF-1α levels peaked at 6 h following I/R, subsequently decreasing to low levels. The levels of downstream effectors peaked at 48 h, and decreased almost to pre-I/R levels by 72 h. These results suggest that HIF-1α and its downstream targets offer only short-term protection following I/R. These results may have implications for the treatment of I/R-associated injury in a variety of clinical contexts.
缺氧诱导因子-1（HIF-1α）在保护心脏组织免受损伤方面发挥着重要作用，例如在缺血再灌注（I/R）后，尽管其发挥作用的时间框架尚未完全阐明。在本研究中，使用来自Sprague-Dawley大鼠的心肌细胞培养模型来检测I/R后不同时间点HIF-1α和各种下游效应物的表达水平。通过在I/R之前过表达HIF-1α来调控HIF-1α的水平。HIF-1α水平在I/R后6小时达到峰值，随后降至低水平。下游效应物的水平在48小时达到峰值，并在72小时时几乎降至I/R前水平。这些结果表明，HIF-1α及其下游靶点在I/R后仅提供短期保护。这些结果可能对多种临床情况下I/R相关损伤的治疗具有启示意义。
