Weerasinghe Harshini, Payne Michael, Beard Sally, Andrianopoulos Alex
Genetics, Genomics & Development, School of BioSciences, University of Melbourne, Victoria 3010, Australia.
Future Microbiol. 2016;11(4):511-26. doi: 10.2217/fmb.16.9. Epub 2016 Apr 13.
Organism-wide approaches examining the genetic mechanisms controlling growth and proliferation have proven to be a powerful tool in the study of pathogenic fungi. For many fungal pathogens techniques to study transcription and protein expression are particularly useful, and offer insights into infection processes by these species. Here we discuss the use of approaches such as differential display, suppression subtractive hybridization, microarray, RNA-seq, proteomics, genetic manipulation and infection models for the AIDS-defining pathogen Talaromyces marneffei. Together these methods have broadened our understanding of the biological processes, and genes that underlie them, which are involved in switching between the saprophytic and pathogenic states of T. marneffei, the maintenance of these two specialized cell types and its ability to cause disease.
研究控制生长和增殖的遗传机制的全生物体方法已被证明是研究致病真菌的有力工具。对于许多真菌病原体而言，研究转录和蛋白质表达的技术特别有用，并能深入了解这些物种的感染过程。在这里，我们讨论了诸如差异显示、抑制性消减杂交、微阵列、RNA测序、蛋白质组学、基因操作和感染模型等方法在艾滋病界定病原体马尔尼菲篮状菌中的应用。这些方法共同拓宽了我们对马尔尼菲篮状菌在腐生和致病状态之间转换、这两种特殊细胞类型的维持及其致病能力所涉及的生物学过程和相关基因的理解。
