Kumar Neeraj, Nahid Fouzia, Zahra Rabaab
a Department of Microbiology , Quaid-i-Azam University , Islamabad , Pakistan.
J Chemother. 2017 Feb;29(1):8-13. doi: 10.1080/1120009X.2016.1154682. Epub 2016 Apr 11.
Escherichia coli, the most frequent cause of UTIs has extensive genetic substructure and can be assigned to eight phylogroups, A, B1, B2, C, D, E, F and Escherichia cryptic clade I. We investigated the distribution of virulence determinants and antimicrobial resistance genes in relation to phylogenetic groups.
A total of 77 E. coli isolates were collected from Civil Hospital Badin, Pakistan. Isolates were assigned phylogroups using quadruplex PCR method, while virulence and antibiotic resistance genes, bla and bla were also detected using PCR.
Thirty-four isolates were assigned to group B2, while 23, 2, 1, 7 and 10 isolates were assigned to F, B1, A/C, clade I/II and negative, respectively. Among virulence genes, prevalence of papC (83%) was highest followed by aer (57%), papGII (16%), papGIII (14%), cnf (9%), hly (5%) and sfa (6%). Of these isolates, 23% and 9% were positive for bla and bla, respectively.
大肠杆菌是尿路感染最常见的病因，具有广泛的遗传亚结构，可分为八个系统发育群，即A、B1、B2、C、D、E、F和大肠杆菌隐秘进化枝I。我们研究了与系统发育群相关的毒力决定因素和抗菌药物耐药基因的分布情况。
从巴基斯坦巴丁市民医院收集了77株大肠杆菌分离株。使用四重PCR方法对分离株进行系统发育群分类，同时也使用PCR检测毒力和抗生素耐药基因bla和bla。
34株分离株被归为B2群，而23株、2株、1株、7株和10株分离株分别被归为F、B1、A/C、进化枝I/II和阴性群。在毒力基因中，papC的流行率最高（83%），其次是aer（57%）、papGII（16%）、papGIII（14%）、cnf（9%）、hly（5%）和sfa（6%）。在这些分离株中，bla和bla的阳性率分别为23%和9%。
