两株鹦鹉热衣原体主要外膜蛋白基因的克隆与序列分析
Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains.
作者信息
Zhang Y X, Morrison S G, Caldwell H D, Baehr W
机构信息
Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
出版信息
Infect Immun. 1989 May;57(5):1621-5. doi: 10.1128/iai.57.5.1621-1625.1989.
Abstract
We cloned and sequenced the gene encoding the major outer membrane protein (MOMP) of two Chlamydia psittaci strains, guinea pig inclusion conjunctivitis (GPIC) strain 1, and meningopneumonitis (Mn) strain Cal-10. Intraspecies alignment of the two C. psittaci MOMP genes revealed 80.6% similarity, and interspecies comparison of C. trachomatis and C. psittaci MOMP genes yielded about 68% similarity. As found previously for C. trachomatis MOMP sequences, stretches of predominantly conserved sequences of GPIC and Mn MOMPs were interrupted by four variable domains whose locations were identical to those of C. trachomatis MOMPs. Seven of eight cysteine residues were found at precisely the same positions in GPIC, Mn, and C. trachomatis MOMPs, emphasizing their importance in structure and function of the protein. Collectively, these results indicate that C. psittaci and C. trachomatis MOMP genes diverged from a common ancestor.
摘要
我们克隆并测序了编码两种鹦鹉热衣原体菌株主要外膜蛋白（MOMP）的基因，这两种菌株分别是豚鼠包涵体结膜炎（GPIC）1型菌株和脑膜肺炎（Mn）Cal - 10菌株。两种鹦鹉热衣原体MOMP基因的种内比对显示相似性为80.6%，沙眼衣原体和鹦鹉热衣原体MOMP基因的种间比较产生了约68%的相似性。如先前在沙眼衣原体MOMP序列中所发现的那样，GPIC和Mn MOMP主要保守序列的片段被四个可变结构域打断，其位置与沙眼衣原体MOMP的可变结构域相同。在GPIC、Mn和沙眼衣原体MOMP中，八个半胱氨酸残基中有七个位于完全相同的位置，这突出了它们在该蛋白质结构和功能中的重要性。总体而言，这些结果表明鹦鹉热衣原体和沙眼衣原体MOMP基因源自一个共同祖先。
相似文献
1
Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains.两株鹦鹉热衣原体主要外膜蛋白基因的克隆与序列分析
Infect Immun. 1989 May;57(5):1621-5. doi: 10.1128/iai.57.5.1621-1625.1989.
2
Cloning and sequence analysis of the major outer membrane protein gene of Chlamydia psittaci 6BC.鹦鹉热衣原体6BC主要外膜蛋白基因的克隆与序列分析
Infect Immun. 1991 Aug;59(8):2853-5. doi: 10.1128/iai.59.8.2853-2855.1991.
3
High-level expression of Chlamydia psittaci major outer membrane protein in COS cells and in skeletal muscles of turkeys.鹦鹉热衣原体主要外膜蛋白在COS细胞和火鸡骨骼肌中的高水平表达。
Infect Immun. 1998 Nov;66(11):5494-500. doi: 10.1128/IAI.66.11.5494-5500.1998.
4
Sequence analysis and lipid modification of the cysteine-rich envelope proteins of Chlamydia psittaci 6BC.鹦鹉热衣原体6BC富含半胱氨酸的包膜蛋白的序列分析和脂质修饰
J Bacteriol. 1991 Jun;173(12):3821-30. doi: 10.1128/jb.173.12.3821-3830.1991.
5
Virulence of feline Chlamydia psittaci in mice is not a function of the major outer membrane protein (MOMP).猫衣原体在小鼠中的毒力并非主要外膜蛋白（MOMP）的作用。
Vet Microbiol. 1996 Dec;53(3-4):355-68. doi: 10.1016/s0378-1135(96)01257-6.
6
Sequence analysis of the major outer membrane protein gene of Chlamydia pneumoniae.肺炎衣原体主要外膜蛋白基因的序列分析
Infect Immun. 1991 Jun;59(6):2195-9. doi: 10.1128/iai.59.6.2195-2199.1991.
7
Lack of allelic polymorphism for the major outer membrane protein gene of the agent of guinea pig inclusion conjunctivitis (Chlamydia psittaci).豚鼠包涵体结膜炎病原体（鹦鹉热衣原体）主要外膜蛋白基因缺乏等位基因多态性。
Infect Immun. 1993 Jul;61(7):3078-80. doi: 10.1128/iai.61.7.3078-3080.1993.
8
Dissociation of immune determinants of outer membrane proteins of Chlamydia psittaci strain guinea pig inclusion conjunctivitis.鹦鹉热衣原体豚鼠包涵体结膜炎菌株外膜蛋白免疫决定簇的解离
Infect Immun. 1994 Dec;62(12):5614-23. doi: 10.1128/iai.62.12.5614-5623.1994.
9
Comparison of the major outer-membrane protein (MOMP) gene of mouse pneumonitis (MoPn) and hamster SFPD strains of Chlamydia trachomatis with other Chlamydia strains.沙眼衣原体小鼠肺炎（MoPn）株和仓鼠SFPD株的主要外膜蛋白（MOMP）基因与其他衣原体菌株的比较。
Mol Biol Evol. 1993 Nov;10(6):1327-42. doi: 10.1093/oxfordjournals.molbev.a040079.
10
Remarkable sequence relatedness in the DNA encoding the major outer membrane protein of Chlamydia psittaci (koala type I) and Chlamydia pneumoniae.鹦鹉热衣原体（考拉I型）和肺炎衣原体主要外膜蛋白编码DNA中的显著序列相关性。
Gene. 1994 Jan 28;138(1-2):139-42. doi: 10.1016/0378-1119(94)90796-x.
引用本文的文献
1
Molecular characterization of species in commercial and backyard poultry farms in Costa Rica.哥斯达黎加商业和家庭式家禽养殖场中物种的分子特征分析。
Epidemiol Infect. 2022 Feb 24;150:1-18. doi: 10.1017/S0950268821002715.
2
Molecular identification and successful treatment of (genotype B) in a clinically affected Congo African grey parrot ().一只临床发病的刚果非洲灰鹦鹉（）中（基因型B）的分子鉴定及成功治疗
Iran J Vet Res. 2016 Fall;17(4):281-285.
3
Chlamydia psittaci genotype B in a pigeon (Columba livia) inhabiting a public place in San José, Costa Rica.在哥斯达黎加圣何塞一个公共场所栖息的一只鸽子（家鸽）体内发现鹦鹉热衣原体B基因型。
Open Vet J. 2013;3(2):135-9. Epub 2013 Dec 2.
4
Molecular Detection and Genotyping of Chlamydia psittaci in Captive Psittacines from Costa Rica.哥斯达黎加圈养鹦鹉中鹦鹉热衣原体的分子检测与基因分型
Vet Med Int. 2013;2013:142962. doi: 10.1155/2013/142962. Epub 2013 Sep 18.
5
Genotyping of Chlamydophila psittaci strains derived from avian and mammalian species.对来源于鸟类和哺乳动物物种的鹦鹉热衣原体菌株进行基因分型。
Vet Res Commun. 2009 Aug;33(6):577-80. doi: 10.1007/s11259-008-9198-8. Epub 2009 Jan 13.
6
Sequencing of the Chlamydophila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method.鹦鹉热嗜衣原体ompA基因测序揭示了一种新的基因型E/B，以及对快速鉴别基因分型方法的需求。
J Clin Microbiol. 2005 May;43(5):2456-61. doi: 10.1128/JCM.43.5.2456-2461.2005.
7
Recombination in the ompA gene but not the omcB gene of Chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism.衣原体ompA基因而非omcB基因的重组导致了该生物体在组织嗜性、免疫监测和持续性方面的血清型特异性差异。
J Bacteriol. 2001 Oct;183(20):5997-6008. doi: 10.1128/JB.183.20.5997-6008.2001.
8
Chlamydia pneumoniae major outer membrane protein is a surface-exposed antigen that elicits antibodies primarily directed against conformation-dependent determinants.肺炎衣原体主要外膜蛋白是一种表面暴露抗原，可引发主要针对构象依赖性决定簇的抗体。
Infect Immun. 2001 May;69(5):3082-91. doi: 10.1128/IAI.69.5.3082-3091.2001.
9
Expression of Chlamydia psittaci- and human immunodeficiency virus-derived antigens on the cell surface of Lactobacillus fermentum BR11 as fusions to bspA.鹦鹉热衣原体和人类免疫缺陷病毒衍生抗原作为与bspA的融合蛋白在发酵乳杆菌BR11细胞表面的表达。
Infect Immun. 1999 Oct;67(10):5486-9. doi: 10.1128/IAI.67.10.5486-5489.1999.
10
High-level expression of Chlamydia psittaci major outer membrane protein in COS cells and in skeletal muscles of turkeys.鹦鹉热衣原体主要外膜蛋白在COS细胞和火鸡骨骼肌中的高水平表达。
Infect Immun. 1998 Nov;66(11):5494-500. doi: 10.1128/IAI.66.11.5494-5500.1998.
本文引用的文献
1
GUINEA PIG INCLUSION CONJUNCTIVITIS VIRUS. I. ISOLATION AND IDENTIFICATION AS A MEMBER OF THE PSITTACOSIS-LYMPHOGRANULOMA-TRACHOMA GROUP.豚鼠包涵体结膜炎病毒。I. 作为鹦鹉热-淋巴肉芽肿-沙眼病毒群成员的分离与鉴定
J Infect Dis. 1964 Feb;114:1-12. doi: 10.1093/infdis/114.1.1.
2
Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis.沙眼衣原体主要外膜蛋白的纯化及部分特性分析
Infect Immun. 1981 Mar;31(3):1161-76. doi: 10.1128/iai.31.3.1161-1176.1981.
3
Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization.抗沙眼衣原体单克隆抗体：抗体特异性及抗原特性
J Immunol. 1982 Mar;128(3):1083-9.
4
Chlamydiae.衣原体
Annu Rev Microbiol. 1980;34:285-309. doi: 10.1146/annurev.mi.34.100180.001441.
5
Disulfide-linked oligomers of the major outer membrane protein of chlamydiae.衣原体主要外膜蛋白的二硫键连接寡聚体。
J Bacteriol. 1983 May;154(2):998-1001. doi: 10.1128/jb.154.2.998-1001.1983.
6
Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis.二硫键在沙眼衣原体外膜结构及通透性中的作用
Infect Immun. 1984 May;44(2):479-85. doi: 10.1128/iai.44.2.479-485.1984.
7
Structural and polypeptide differences between envelopes of infective and reproductive life cycle forms of Chlamydia spp.衣原体属感染性和繁殖性生命周期形式的包膜之间的结构和多肽差异
J Bacteriol. 1984 Jan;157(1):13-20. doi: 10.1128/jb.157.1.13-20.1984.
8
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
9
Lack of deoxyribonucleic acid homology between species of the genus Chlamydia.衣原体属各物种之间缺乏脱氧核糖核酸同源性。
J Bacteriol. 1968 Oct;96(4):1421-3. doi: 10.1128/jb.96.4.1421-1423.1968.
10
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.超螺旋测序：一种用于质粒DNA测序的快速简便方法。
DNA. 1985 Apr;4(2):165-70. doi: 10.1089/dna.1985.4.165.
Zotero 插件