Laboratory of virus contaminants of water and food. Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Catalonia, Spain.
Center for Pathophysiology, Infectiology and Immunology, Institute for Hygiene and Applied Immunology, Water Hygiene, Medical University Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria.
Int J Hyg Environ Health. 2016 Jul;219(4-5):405-11. doi: 10.1016/j.ijheh.2016.04.002. Epub 2016 Apr 8.
Hepatitis E Virus (HEV) is a major cause of waterborne outbreaks in areas with poor sanitation. As safe water supplies are the keystone for preventing HEV outbreaks, data on the efficacy of disinfection treatments are urgently needed. Here, we evaluated the ability of UV radiation and flocculation-chlorination sachets (FCSs) to reduce HEV in water matrices. The HEV-p6-kernow strain was replicated in the HepG2/C3A cell line, and we quantified genome number using qRT-PCR and infectivity using an immunofluorescence assay (IFA). UV irradiation tests using low-pressure radiation showed inactivation kinetics for HEV of 99.99% with a UV fluence of 232J/m(2) (IC 95%, 195,02-269,18). Moreover, the FCSs preparations significantly reduced viral concentrations in both water matrices, although the inactivation results were under the baseline of reduction (4.5 LRV) proposed by WHO guidelines.
戊型肝炎病毒（HEV）是卫生条件较差地区水传播暴发的主要原因。由于安全供水是预防 HEV 暴发的关键，因此迫切需要有关消毒处理功效的数据。在这里，我们评估了紫外线辐射和絮凝-氯化小袋（FCS）在水基质中降低 HEV 的能力。HEV-p6-kernow 株在 HepG2/C3A 细胞系中复制，我们使用 qRT-PCR 定量基因组数量，并使用免疫荧光测定法（IFA）测定感染性。使用低压辐射进行的紫外线照射测试表明，紫外线辐照 232J/m(2)（IC95%，195.02-269.18）时，HEV 的灭活动力学达到 99.99%。此外，FCS 制剂在两种水基质中均显著降低了病毒浓度，尽管灭活结果低于世界卫生组织指南提出的（4.5LRV）的减少基准。
