Girones Rosina, Carratalà Anna, Calgua Byron, Calvo Miquel, Rodriguez-Manzano Jesús, Emerson Suzanne
Department of Microbiology, Faculty of Biology, University of Barcelona, Avd. Diagonal 643, Barcelona, 08028, Catalonia, Spain E-mail:
Department of Biostatistics, University of Barcelona, Barcelona, 08028, Spain.
J Water Health. 2014 Sep;12(3):436-42. doi: 10.2166/wh.2014.027.
Hepatitis E virus (HEV) is transmitted via the fecal-oral route and has been recognized as a common source of large waterborne outbreaks involving contaminated water in developing countries. Thus, there is the need to produce experimental data on the disinfection kinetics of HEV by chlorine in water samples with diverse levels of fecal contamination. Here, the inactivation of HEV and human adenovirus C serotype 2 (HAdV2), used as a reference virus, was monitored using immunofluorescence and quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays. HEV has been shown to be susceptible to chlorine disinfection and presented equivalent kinetics to human adenoviruses. The C(t) values observed for a 2-log reduction of HEV were 0.41 in buffered demand-free water and 11.21 mg/L × min in the presence of 1% sewage. The results indicate that the inactivation kinetics of HEV and HAdV2 are equivalent and support the use of chlorine disinfection as an effective strategy to control HEV waterborne transmission.
戊型肝炎病毒（HEV）通过粪-口途径传播，在发展中国家已被确认为涉及受污染水源的大规模水源性疫情的常见源头。因此，有必要生成关于不同粪便污染水平水样中氯对HEV消毒动力学的实验数据。在此，使用免疫荧光和定量逆转录聚合酶链反应（RT-qPCR）测定法监测了HEV和用作参考病毒的人类腺病毒C血清型2（HAdV2）的灭活情况。已证明HEV对氯消毒敏感，且其动力学与人类腺病毒相当。在无需求缓冲水中，使HEV减少2个对数级观察到的C(t)值为0.41，在存在1%污水的情况下为11.21 mg/L×分钟。结果表明，HEV和HAdV2的灭活动力学相当，并支持使用氯消毒作为控制HEV水源性传播的有效策略。
