Yasmeen Farhat, Raja Naveed Iqbal, Mustafa Ghazala, Sakata Katsumi, Komatsu Setsuko
Department of Botany, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan; National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
Department of Botany, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan.
J Proteomics. 2016 Jun 30;143:136-150. doi: 10.1016/j.jprot.2016.03.014. Epub 2016 Apr 11.
Aluminum oxide nanoparticles (Al2O3 NPs) are used in various commercial and agricultural products. Soybean exhibits severe reduction in growth under flooding condition. To examine the effects of Al2O3 NPs on the recovery of soybean from flooding, proteomic analysis was performed. Survival percentage and weight/length of root including hypocotyl were improved after 2 and 4days of flooding with 50ppm Al2O3 NPs leading to recovery as compared to flooding. A total of 211 common proteins were changed in abundance during the recovery period after treatment without or with Al2O3 NPs. These proteins were related to protein synthesis, stress, cell wall, and signaling. Among the identified stress-related proteins, S-adenosyl-l-methionine dependent methyltransferases were recovered from flooding with Al2O3 NPs. Hierarchical clustering divided the identified proteins into three clusters. Cluster II exhibited the greatest change in proteins related to protein synthesis, transport, and development during the recovery from flooding with Al2O3 NPs. However, activity of enolase remained unchanged during flooding leading to subsequent recovery with Al2O3 NPs. These results suggest that S-adenosyl-l-methionine dependent methyltransferases and enolase might be involved in mediating recovery responses by Al2O3 NPs.
This study highlighted the role of Al2O3 NPs in recovery of soybean seedlings from flooding stress using gel-free proteomic technique. The key findings of this study are as follows: (i) survival percentage was enhanced at 50ppm Al2O3 NPs during the recovery stage; (ii) seedling weight and weight/length of root including hypocotyl improved at 50ppm Al2O3 NPs during the period of recovery; (iii) protein synthesis and stress related proteins were increased on recovery after flooding without or with Al2O3 NPs; (iv) the abundance of S-adenosyl-l-methionine dependent methyltransferases recovered from flooding with Al2O3 NPs; (v) glycolysis related proteins amplified under flooding with Al2O3 NPs; (vi) enolase enzyme remained unchanged during flooding leading to subsequent recovery from flooding with Al2O3 NPs. Collectively, these results suggest that S-adenosyl-l-methionine dependent methyltransferases and enolase are involved in response to flooding with Al2O3 NPs and might be helpful in recovery from flooding stress.
氧化铝纳米颗粒（Al2O3 NPs）被用于各种商业和农业产品中。大豆在淹水条件下生长会严重受抑制。为了研究Al2O3 NPs对大豆从淹水中恢复的影响，进行了蛋白质组学分析。与淹水处理相比，用50ppm Al2O3 NPs进行2天和4天淹水处理后，大豆的存活率以及包括下胚轴在内的根的重量/长度得到改善，从而实现恢复。在处理后（无论有无Al2O3 NPs）的恢复期间，共有211种常见蛋白质的丰度发生了变化。这些蛋白质与蛋白质合成、应激、细胞壁和信号传导有关。在鉴定出的与应激相关的蛋白质中，依赖S -腺苷 - L -甲硫氨酸的甲基转移酶在Al2O3 NPs淹水处理后恢复。层次聚类将鉴定出的蛋白质分为三个簇。在从Al2O3 NPs淹水中恢复的过程中，簇II中与蛋白质合成、运输和发育相关的蛋白质变化最大。然而，烯醇化酶的活性在淹水期间保持不变，随后在Al2O3 NPs处理后恢复。这些结果表明，依赖S -腺苷 - L -甲硫氨酸的甲基转移酶和烯醇化酶可能参与介导Al2O3 NPs的恢复反应。
本研究利用无凝胶蛋白质组学技术突出了Al2O3 NPs在大豆幼苗从淹水胁迫中恢复过程中的作用。本研究的主要发现如下：（i）在恢复阶段，50ppm Al2O3 NPs处理可提高存活率；（ii）在恢复期间，50ppm Al2O3 NPs处理可提高幼苗重量以及包括下胚轴在内的根的重量/长度；（iii）淹水后无论有无Al2O3 NPs处理，恢复过程中蛋白质合成和应激相关蛋白质均增加；（iv）依赖S -腺苷 - L -甲硫氨酸的甲基转移酶的丰度在Al2O3 NPs淹水处理后恢复；（v）在Al2O3 NPs淹水处理下糖酵解相关蛋白质增加；（vi）烯醇化酶在淹水期间保持不变，随后在Al2O3 NPs淹水处理后恢复。总体而言，这些结果表明依赖S -腺苷 - L -甲硫氨酸的甲基转移酶和烯醇化酶参与了对Al2O3 NPs淹水的反应，可能有助于从淹水胁迫中恢复。
