Faculty of Life and Environmental and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan.
Department of Agronomy, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan.
Int J Mol Sci. 2019 Feb 14;20(4):825. doi: 10.3390/ijms20040825.
Production and utilization of nanoparticles (NPs) are increasing due to their positive and stimulating effects on biological systems. Silver (Ag) NPs improve seed germination, photosynthetic efficiency, plant growth, and antimicrobial activities. In this study, the effects of chemo-blended Ag NPs on wheat were investigated using the gel-free/label-free proteomic technique. Morphological analysis revealed that chemo-blended Ag NPs resulted in the increase of shoot length, shoot fresh weight, root length, and root fresh weight. Proteomic analysis indicated that proteins related to photosynthesis and protein synthesis were increased, while glycolysis, signaling, and cell wall related proteins were decreased. Proteins related to redox and mitochondrial electron transport chain were also decreased. Glycolysis associated proteins such as glyceraldehyde-3-phosphate dehydrogenase increased as well as decreased, while phosphoenol pyruvate carboxylase was decreased. Antioxidant enzyme activities such as superoxide dismutase, catalase, and peroxidase were promoted in response to the chemo-blended Ag NPs. These results suggested that chemo-blended Ag NPs promoted plant growth and development through regulation of energy metabolism by suppression of glycolysis. Number of grains/spike, 100-grains weight, and yield of wheat were stimulated with chemo-blended Ag NPs. Morphological study of next generational wheat plants depicted normal growth, and no toxic effects were observed. Therefore, morphological, proteomic, yield, and next generation results revealed that chemo-blended Ag NPs may promote plant growth and development through alteration in plant metabolism.
由于纳米粒子 (NPs) 对生物系统具有积极的刺激作用，其生产和利用正在增加。银 (Ag) NPs 可提高种子发芽率、光合作用效率、植物生长和抗菌活性。在这项研究中，使用无胶/无标记的蛋白质组学技术研究了化学混合 Ag NPs 对小麦的影响。形态分析表明，化学混合 Ag NPs 导致芽长、芽鲜重、根长和根鲜重增加。蛋白质组学分析表明，与光合作用和蛋白质合成相关的蛋白质增加，而糖酵解、信号和细胞壁相关的蛋白质减少。与氧化还原和线粒体电子传递链相关的蛋白质也减少。与糖酵解相关的蛋白质如 3-磷酸甘油醛脱氢酶增加和减少，而磷酸烯醇丙酮酸羧激酶减少。超氧化物歧化酶、过氧化氢酶和过氧化物酶等抗氧化酶活性被促进，以响应化学混合 Ag NPs。这些结果表明，化学混合 Ag NPs 通过抑制糖酵解来调节能量代谢，从而促进植物的生长和发育。用化学混合 Ag NPs 刺激小麦的穗粒数/穗、百粒重和产量。下一代小麦植物的形态研究显示正常生长，未观察到有毒作用。因此，形态学、蛋白质组学、产量和下一代结果表明，化学混合 Ag NPs 可能通过改变植物代谢来促进植物的生长和发育。
