Lan Zhou, Wei Meng, Chen Lvyi, Xie Guangjing, Liu Xiao, Zhang Xiuqiao
School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, People's Republic of China.
School of Pharmacy, South-Central University for Nationalities, Wuhan, People's Republic of China.
IUBMB Life. 2016 Jun;68(6):429-35. doi: 10.1002/iub.1499. Epub 2016 Apr 15.
The investigation was undertaken to evaluate the effect of sinomenine (Sin) on experimental adjuvant arthritis rats stimulated by Freund's complete adjuvant and explore the corresponding potential molecular mechanism. The content of proinflammatory cytokines such as tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 were detected. Besides, canonical nuclear factor kappa B (NF-κB) pathway was also assessed to evaluate the antiarthritic potential of sinomenine. Pathological sections of rat paws showed sinomenine and diclofenac sodium significantly alleviated articular cartilage lesion, cellular infiltration, epithelial cell degeneration, synovial tissue vasodilation and congestion. The phosphorylations of inhibitor of kappaB alpha and NF-κB subunit p65 were downregulated with the treatment of sinomenine in dose dependent manners, as well as proinflammatory cytokines. Therefore, it was assumed that sinomenine might be a new therapeutic candidate to treat arthritis. © 2016 IUBMB Life, 68(6):429-435, 2016.
本研究旨在评估青藤碱（Sin）对弗氏完全佐剂刺激的实验性佐剂性关节炎大鼠的影响，并探索相应的潜在分子机制。检测了肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6等促炎细胞因子的含量。此外，还评估了经典的核因子κB（NF-κB）通路，以评估青藤碱的抗关节炎潜力。大鼠爪部病理切片显示，青藤碱和双氯芬酸钠显著减轻了关节软骨损伤、细胞浸润、上皮细胞变性、滑膜组织血管扩张和充血。随着青藤碱剂量依赖性治疗，κBα抑制因子和NF-κB亚基p65的磷酸化以及促炎细胞因子均下调。因此，推测青藤碱可能是治疗关节炎的一种新的候选治疗药物。© 2016国际生物化学与分子生物学联盟生命科学，68(6):429 - 435，2016。
