Nugraha Alexander Patera, Triwardhani Ari, Sitalaksmi Ratri Maya, Ramadhani Nastiti Faradilla, Luthfi Muhammad, Ulfa Ninik Mas, Tengku Ahmad Noor Tengku Natasha Eleena Binti
Orthodontics Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Prosthodontics Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
J Oral Biol Craniofac Res. 2023 Nov-Dec;13(6):720-726. doi: 10.1016/j.jobcr.2023.09.004. Epub 2023 Sep 18.
the leaf (MO) has active compounds that may be beneficial for peri-implantitis therapy. This research aims to analyze the phytochemical, antioxidant, and antibacterial properties of L. nanosuspension (MON) extract in peri-implantitis-related bacteria.
MON extract phytochemical analysis was conducted to examine active compounds such as flavonoids, saponins, quinones, alkaloids, tannins, terpenoids, and steroids. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay for antioxidant capacity was evaluated, and gas chromatography-mass spectrometry for the detection of volatile active compounds in MON extract was performed. Turax was used to create MON extract at concentrations of 1% and 2%, and then a particle size analysis was carried out. (Pi), (Pg), (Aa), and (Fn) were tested for antibacterial activity of MON extract, comparing them with doxycycline as the reference drug and using the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and diffusion zone methods.
MON extract has lower antioxidant capacity than vitamin C. Flavonoids, saponins, quinones, alkaloids, tannins, terpenoids, and steroids were found in MON extract. 1% and 2% of MON extract has 10-40 d nm particle size. MIC, MBC and diffusion examination of 1% and 2% MON extract on Aa, Pg, Pi, and Fn were seen at concentrations of 25% and 12.5% with significantly different () in vitro.
MON extract has potential antioxidant activity, and 1% or 2% of MON extract has antibacterial properties toward Aa, Pg, Pi, and Fn at concentrations of 25% and 12.5%, with significant differences.
月桂叶（MO）含有活性化合物，可能对种植体周围炎治疗有益。本研究旨在分析月桂叶纳米悬浮液（MON）提取物对种植体周围炎相关细菌的植物化学、抗氧化和抗菌特性。
对MON提取物进行植物化学分析，以检测黄酮类、皂苷类、醌类、生物碱类、单宁类、萜类和甾体类等活性化合物。评估2,2-二苯基-1-苦基肼（DPPH）抗氧化能力测定法，并进行气相色谱-质谱联用检测MON提取物中的挥发性活性化合物。用吐温80制备浓度为1%和2%的MON提取物，然后进行粒度分析。测试牙龈卟啉单胞菌（Pg）、具核梭杆菌（Fn）、伴放线聚集杆菌（Aa）和中间普氏菌（Pi）对MON提取物的抗菌活性，以强力霉素作为参考药物，采用最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和扩散区法进行比较。
MON提取物的抗氧化能力低于维生素C。在MON提取物中发现了黄酮类、皂苷类、醌类、生物碱类、单宁类、萜类和甾体类。1%和2%的MON提取物粒径为10 - 40 d nm。1%和2%的MON提取物对Aa、Pg、Pi和Fn的MIC、MBC和扩散检测在浓度为25%和12.5%时可见，体外有显著差异（）。
MON提取物具有潜在的抗氧化活性，1%或2%的MON提取物在浓度为25%和12.5%时对Aa、Pg、Pi和Fn具有抗菌特性，差异显著。
