Slobodkina Galina B, Kolganova Tatyana V, Kopitsyn Dmitry S, Viryasov Mikhail B, Bonch-Osmolovskaya Elizaveta A, Slobodkin Alexander I
Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect 33, bld. 2, 119071 Moscow, Russia.
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy 33, bld. 2, 119071 Moscow, Russia.
Int J Syst Evol Microbiol. 2016 Jul;66(7):2515-2519. doi: 10.1099/ijsem.0.001083. Epub 2016 Apr 15.
A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain SH388, was isolated from a shallow, submarine hydrothermal vent (Kuril Islands, Russia). Cells of strain SH388 were Gram-stain-negative short rods, 0.2-0.4 µm in diameter and 1.0-2.5 µm in length, and motile with flagella. The temperature range for growth was 25-58 °C (optimum 50 °C), and the pH range for growth was pH 5.0-7.0 (optimum pH 6.0-6.5). Growth of strain SH388 was observed in the presence of NaCl concentrations ranging from 0.5 to 4.0 % (w/v) (optimum 2.0-2.5 %). The strain grew chemolithoautotrophically with molecular hydrogen as electron donor, sodium sulfite as electron acceptor and bicarbonate/CO2 as a carbon source. It was also able to grow by disproportionation of sulfite and elemental sulfur but not thiosulfate. Sulfate, Fe(III) and nitrate were not used as electron acceptors either with H2 or organic electron donors. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the class Deltaproteobacteria and was most closely related to Dissulfuribacter thermophilus and Dissulfurimicrobium hydrothermale (91.6 % and 90.4 % sequence similarity). On the basis of its physiological properties and results of phylogenetic analyses, strain SH388 is considered to represent a novel species of a new genus, for which the name Dissulfurirhabdus thermomarina gen. nov., sp. nov. is proposed. The type strain of the species is SH388 (=DSM 100025=VKM B-2960). It is the first thermophilic disproportionator of sulfur compounds isolated from a shallow-sea environment.
从俄罗斯千岛群岛一处浅海海底热液喷口分离出一株嗜热、厌氧、化能无机自养细菌，菌株SH388。菌株SH388的细胞为革兰氏阴性短杆菌，直径0.2 - 0.4μm，长度1.0 - 2.5μm，具鞭毛，能运动。生长温度范围为25 - 58°C（最适温度50°C），生长pH范围为pH 5.0 - 7.0（最适pH 6.0 - 6.5）。在NaCl浓度为0.5%至4.0%（w/v）（最适浓度2.0 - 2.5%）的条件下可观察到菌株SH388生长。该菌株以分子氢作为电子供体、亚硫酸钠作为电子受体、碳酸氢盐/二氧化碳作为碳源进行化能无机自养生长。它也能够通过亚硫酸盐和元素硫的歧化作用生长，但不能利用硫代硫酸盐。无论是以氢气还是有机电子供体作为电子供体时，硫酸盐、Fe(III)和硝酸盐都不能用作电子受体。基于16S rRNA基因序列的系统发育分析表明，该分离株属于δ-变形菌纲，与嗜热双硫还原菌（Dissulfuribacter thermophilus）和热液双硫微菌（Dissulfurimicrobium hydrothermale）关系最为密切（序列相似性分别为91.6%和90.4%）。基于其生理特性和系统发育分析结果，菌株SH388被认为代表了一个新属的新物种，为此提出新属名热海双硫杆状菌属（Dissulfurirhabdus），新种名热海双硫杆状菌（Dissulfurirhabdus thermomarina）。该物种的模式菌株为SH388（=DSM 100025=VKM B - 2960）。它是从浅海环境中分离出的首例嗜热硫化合物歧化菌。
