de Almeida M, Gazagne I, Jeulin C, Herry M, Belaisch-Allart J, Frydman R, Jouannet P, Testart J
Laboratoire d'Histologie Embryologie et Cytogénétique, Centre Hospitalier de Bicêtre, France.
Hum Reprod. 1989 Jan;4(1):49-53. doi: 10.1093/oxfordjournals.humrep.a136844.
In-vitro fertilization and embryo transfer were carried out in twenty infertile couples with significant levels of anti-sperm antibodies on the ejaculated spermatozoa. Over 21 cycles (15 couples), 95 mature oocytes were collected and inseminated with spermatozoa obtained by swim-up migration after rapid dilution and washing of the ejaculates. An overall fertilization rate of 38.9% was obtained with these post-migration (PM) preparations. When greater than 70% of the inseminated spermatozoa were covered with both IgG and IgA antibodies, only 14% of the 43 oocytes were fertilized. A higher fertilization rate was obtained with the PM preparations containing less than 70% of spermatozoa coated with one or both classes of antibodies. Under these conditions 60% of the 52 oocytes were fertilized. Fertility rate correlated better with IgG than with IgA antibody levels. In order to decrease the proportion of antibody-coated spermatozoa in the inseminated populations, washed spermatozoa were immuno-adsorbed on Mage's plates before swim-up migration. Over 11 cycles (10 couples) 52 mature oocytes were inseminated with these post-migration immuno-depleted sperm preparations (PMP) containing less than 65% of antibody-coated spermatozoa: 31% of the oocytes were fertilized. This rate compares favourably to IVF results obtained with the PM preparations with greater than 70% of spermatozoa coated with both classes of antibodies. In five couples who had different IVF attempts with the two sperm preparations, the immuno-depletion resulted in a slight increase in the fertilization rates: 10% for the PM preparations versus 26% for the PMP preparations. Sperm binding to the zona pellucida was decreased in the majority of unsuccessful attempts.(ABSTRACT TRUNCATED AT 250 WORDS)
对20对不育夫妇进行体外受精和胚胎移植，这些夫妇射出的精子上抗精子抗体水平较高。在超过21个周期（15对夫妇）中，收集了95个成熟卵母细胞，并用经快速稀释和洗涤后的射精物通过上浮迁移获得的精子进行授精。这些迁移后（PM）制剂的总体受精率为38.9%。当超过70%的授精精子同时被IgG和IgA抗体覆盖时，43个卵母细胞中只有14%受精。对于含有少于70%被一类或两类抗体包被的精子的PM制剂，受精率更高。在这些条件下，52个卵母细胞中有60%受精。受精率与IgG抗体水平的相关性比与IgA抗体水平的相关性更好。为了减少授精群体中被抗体包被的精子比例，在精子上浮迁移前，将洗涤后的精子免疫吸附在梅奇板上。在超过11个周期（10对夫妇）中，用这些含有少于65%被抗体包被的精子的迁移后免疫去除精子制剂（PMP）对52个成熟卵母细胞进行授精：31%的卵母细胞受精。该受精率优于用超过70%被两类抗体包被的精子的PM制剂获得的体外受精结果。在5对夫妇中，分别用这两种精子制剂进行了不同的体外受精尝试，免疫去除导致受精率略有提高：PM制剂为10%，而PMP制剂为26%。在大多数未成功的尝试中，精子与透明带的结合减少。（摘要截选至250词）
