Jeulin C, Soumah A, Da Silva G, De Almeida M
Laboratoire d'Histologie-Embryologie, Centre Hospitalier Universitaire, France.
Hum Reprod. 1989 Jan;4(1):44-8. doi: 10.1093/oxfordjournals.humrep.a136843.
Ejaculates from 51 infertile men with significant levels of anti-sperm antibodies were submitted to processing in vitro in order to increase the number of antibody-free spermatozoa. Rapid removal and washing of spermatozoa from antibody-containing seminal fluid resulted in a variable decrease in IgG and/or IgA binding; only IgG binding was significantly reduced. Those spermatozoa were allowed to swim-up into an overlaying medium to select those with the best progression. Unexpectedly, in the post-migration samples (PM), the proportion of progressively motile spermatozoa coated with IgG and IgA antibodies increased significantly. An efficient selection of antibody-free spermatozoa was achieved prior to swim up migration by immuno-binding to polystyrene Petri plates coated with anti-human immunoglobulin antibodies. Non-adherent populations were depleted of 27-100% of IgG and IgA antibody-coated spermatozoa. After migration, the percentages of antibody-coated spermatozoa of those populations (PMP) remained low. The comparison between PM and PMP populations shows that immunobinding increases the number of motile antibody-free spermatozoa. The fertility potential of both sperm populations is currently under investigation.
对51名抗精子抗体水平较高的不育男性的精液进行体外处理，以增加无抗体精子的数量。从含抗体的精液中快速去除并洗涤精子，导致IgG和/或IgA结合出现不同程度的下降；只有IgG结合显著降低。让这些精子游入上层培养基中，以选择前进能力最佳的精子。出乎意料的是，在迁移后样本（PM）中，被IgG和IgA抗体包被的进行性运动精子的比例显著增加。在精子向上迁移之前，通过与包被抗人免疫球蛋白抗体的聚苯乙烯培养皿进行免疫结合，实现了对无抗体精子的有效筛选。非黏附群体中27%-100%的IgG和IgA抗体包被的精子被去除。迁移后，这些群体（PMP）中抗体包被精子的百分比仍然很低。PM群体和PMP群体之间的比较表明，免疫结合增加了可运动的无抗体精子的数量。目前正在研究这两种精子群体的生育潜力。
