Marino Daniel, Ariz Idoia, Lasa Berta, Santamaría Enrique, Fernández-Irigoyen Joaquín, González-Murua Carmen, Aparicio Tejo Pedro M
Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080 Bilbao, Spain Ikerbasque, Basque Foundation for Science, E-48011 Bilbao, Spain
Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Spain Faculdade de Ciências, Centro Ecologia Evolução e Alterações Ambientais, Universidade de Lisboa, Lisboa, Portugal.
J Exp Bot. 2016 May;67(11):3313-23. doi: 10.1093/jxb/erw147. Epub 2016 Apr 16.
Accessing different nitrogen (N) sources involves a profound adaptation of plant metabolism. In this study, a quantitative proteomic approach was used to further understand how the model plant Arabidopsis thaliana adjusts to different N sources when grown exclusively under nitrate or ammonium nutrition. Proteome data evidenced that glucosinolate metabolism was differentially regulated by the N source and that both TGG1 and TGG2 myrosinases were more abundant under ammonium nutrition, which is generally considered to be a stressful situation. Moreover, Arabidopsis plants displayed glucosinolate accumulation and induced myrosinase activity under ammonium nutrition. Interestingly, these results were also confirmed in the economically important crop broccoli (Brassica oleracea var. italica). Moreover, these metabolic changes were correlated in Arabidopsis with the differential expression of genes from the aliphatic glucosinolate metabolic pathway. This study underlines the importance of nitrogen nutrition and the potential of using ammonium as the N source in order to stimulate glucosinolate metabolism, which may have important applications not only in terms of reducing pesticide use, but also for increasing plants' nutritional value.
利用不同的氮源需要植物代谢进行深刻的适应性调整。在本研究中，采用定量蛋白质组学方法进一步了解模式植物拟南芥在仅以硝酸盐或铵盐为营养源生长时如何适应不同的氮源。蛋白质组数据表明，硫代葡萄糖苷代谢受氮源的差异调节，并且在铵盐营养条件下，TGG1和TGG2这两种黑芥子酶更为丰富，而铵盐营养通常被认为是一种胁迫条件。此外，拟南芥植株在铵盐营养条件下表现出硫代葡萄糖苷积累并诱导黑芥子酶活性。有趣的是，这些结果在经济上重要的作物西兰花（甘蓝变种意大利芥）中也得到了证实。此外，这些代谢变化在拟南芥中与来自脂肪族硫代葡萄糖苷代谢途径的基因差异表达相关。本研究强调了氮素营养的重要性以及利用铵盐作为氮源刺激硫代葡萄糖苷代谢的潜力，这不仅在减少农药使用方面可能具有重要应用，而且在提高植物营养价值方面也可能具有重要应用。
