埃博拉病毒的遗传多样性：埃博拉病毒蛋白的系统发育和计算机模拟结构研究
Genetic diversity in Ebola virus: Phylogenetic and in silico structural studies of Ebola viral proteins.
作者信息
Grifoni Alba, Lo Presti Alessandra, Giovanetti Marta, Montesano Carla, Amicosante Massimo, Colizzi Vittorio, Lai Alessia, Zehender Gianguglielmo, Cella Eleonora, Angeletti Silvia, Ciccozzi Massimo
机构信息
ProxAgen Ltd, Sofia, Bulgaria.
Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy.
出版信息
Asian Pac J Trop Med. 2016 Apr;9(4):337-343. doi: 10.1016/j.apjtm.2016.03.016. Epub 2016 Mar 9.
OBJECTIVE
To explore the genetic diversity and the modification of antibody response in the recent outbreak of Ebola Virus.
METHODS
Sequences retrieved from public databases, the selective pressure analysis and the homology modeling based on the all protein (nucleoprotein, VP35, VP40, soluble glycoprotein, small soluble glycoprotein, VP30, VP24 and polymerase) were used.
RESULTS
Structural proteins VP24, VP30, VP35 and VP40 showed relative conserved sequences making them suitable target candidates for antiviral treatment. On the contrary, nucleoprotein, polymerase and soluble glycoprotein have high mutation frequency.
CONCLUSIONS
Data from this study point out important aspects of Ebola virus sequence variability that for epitope and vaccine design should be considered for appropriate targeting of conserved protein regions.
目的
探究近期埃博拉病毒爆发中的基因多样性及抗体反应的变化。
方法
使用从公共数据库检索的序列、选择性压力分析以及基于所有蛋白质（核蛋白、VP35、VP40、可溶性糖蛋白、小可溶性糖蛋白、VP30、VP24和聚合酶）的同源建模。
结果
结构蛋白VP24、VP30、VP35和VP40显示出相对保守的序列，使其成为抗病毒治疗的合适候选靶点。相反，核蛋白、聚合酶和可溶性糖蛋白具有高突变频率。
结论
本研究数据指出了埃博拉病毒序列变异性的重要方面，在表位和疫苗设计中，应考虑这些方面以适当地靶向保守蛋白区域。
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