Shimabukuro Michio, Sato Hiromi, Izaki Hirofumi, Fukuda Daiju, Uematsu Etsuko, Hirata Yoichiro, Yagi Shusuke, Soeki Takeshi, Sakaue Hiroshi, Kanayama Hiro-Omi, Masuzaki Hiroaki, Sata Masataka
Department of Cardio-Diabetes Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
Department of Cardiovascular Medicine, Institute of Biomedical Sciences, University of Tokushima Graduate School, Tokushima, Japan.
Biofactors. 2016 Jul 8;42(4):397-406. doi: 10.1002/biof.1287. Epub 2016 Apr 18.
Gender difference in obesity-associated cardiovascular complication could be derived from divergent chronic inflammation. We evaluated depot- and gender-specific regulation of the innate immune system in human adipose tissues. Pair samples were obtained from subcutaneous (SAT) and visceral adipose tissue (VAT) during elective surgery (Male: 35; Female: 27). Expressions of pro- and anti-inflammatory adipocytokines were evaluated by semi-quantitative qPCR. Adipose cell-size distribution was obtained from tissue samples fixed in osmium tetroxide and analyzed by Beckman Coulter Multisizer. Levels of adiponectin were higher in SAT and VAT of female than those of male (P < 0.001 and P = 0.011, respectively). NLRP3, IL1β-IL18, TLR2 were comparable in SAT and VAT between genders. However, TLR4 and TLR9 were increased in female SAT and VAT and HMGB1 in female VAT. Levels of adiponectin were not correlated with mean diameter of adipocyte (φ, μm) in SAT and VAT of male, but negatively well correlated in those of female (r = -0.392 and r = -0.616). Such negative correlations were also observed between levels of TLR2, TLR4, and HMGB1 and φ in female. Levels of NLRP3 and IL1β were positively correlated with φ in male, but not in female. In conclusion, Innate signals were differentially expressed in male and female adipose tissues, suggesting that the depot- and gender-specific signals could be related to gender difference in chronic inflammation. © 2016 BioFactors, 42(4):397-406, 2016.
肥胖相关心血管并发症中的性别差异可能源于不同的慢性炎症。我们评估了人类脂肪组织中固有免疫系统的储存部位和性别特异性调节。在择期手术期间从皮下脂肪组织（SAT）和内脏脂肪组织（VAT）获取配对样本（男性：35例；女性：27例）。通过半定量qPCR评估促炎和抗炎脂肪细胞因子的表达。从用四氧化锇固定的组织样本中获得脂肪细胞大小分布，并通过贝克曼库尔特多参数血细胞分析仪进行分析。女性SAT和VAT中脂联素水平高于男性（分别为P < 0.001和P = 0.011）。NLRP3、IL1β - IL18、TLR2在不同性别的SAT和VAT中相当。然而，TLR4和TLR9在女性SAT和VAT中增加，HMGB1在女性VAT中增加。男性SAT和VAT中脂联素水平与脂肪细胞平均直径（φ，μm）不相关，但在女性中呈负相关（r = - 0.392和r = - 0.616）。在女性中，TLR2、TLR4和HMGB1水平与φ之间也观察到这种负相关。NLRP3和IL1β水平在男性中与φ呈正相关，但在女性中不相关。总之，固有信号在男性和女性脂肪组织中差异表达，表明储存部位和性别特异性信号可能与慢性炎症中的性别差异有关。© 2016生物因子，42(4):397 - 406，2016。
