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伏安法和分光光度法测定番樱桃叶提取物的抗氧化活性。

Voltammetric and spectrophotometric determination of antioxidant activity of Eugenia dysenterica DC leaves extracts.

作者信息

Clementino Silva Elton, Garcia Rezende Stefani, Moreira Béda Roanna C Clícia, Pagliarini Balest Aiessa, Cabral Reis Bruna, Dâmaris Silveira, de Souza Gil Eric

机构信息

Faculty of Ceilândia, University of Brasilia. Centro Metropolitano - Conjunto A - Lote 01, Ceilândia, Distrito Federal, Brazil.

Faculty of Pharmacy, Federal University of Goias. Av. Universitária, Setor Universitário, Goiânia, Goiás, Brazil.

出版信息

Pak J Pharm Sci. 2016 Mar;29(2):535-40.

PMID:27087097
Abstract

Eugenia dysenterica DC (cagaiteira) is a native tree from Cerrado biome. Cagaita fruits are known and consumed in natura, mainly by inhabitants from Cerrado. In this study, we evaluated the antioxidant activity of leaves of this plant. For this evaluation we used four methods, the reduction of phosphomolybdenum, scanning by hydrogen peroxide, DPPH radical scavenging assay and determination of electrochemical parameters by differential pulse voltammetry. The results indicate that all extracts from leaves of this species have significant antioxidant potential, following the order: crude ethanol extract CEE) >crude aqueous extract (CAE) >crude hexane extract (CHE). The voltammetric approaches were also applied in order to evaluate the redox behavior of the hydrophilic extracts, as well as of their sub-extracts. Thus, the results suggest the presence of catechol-like polyphenols, which were confirmed by chromatography and phytochemical methods. Voltammetric analysis showed to be a reliable and fast method to determine redox behavior of E. dysenterica extracts.

摘要

番樱桃(Eugenia dysenterica DC,卡盖特拉树)是一种来自塞拉多生物群落的本土树木。卡盖特拉果实为人所知且可直接食用,主要是塞拉多地区的居民食用。在本研究中,我们评估了这种植物叶片的抗氧化活性。为了进行该评估,我们使用了四种方法,即磷钼酸还原法、过氧化氢扫描法、DPPH自由基清除测定法以及通过差分脉冲伏安法测定电化学参数。结果表明,该物种叶片的所有提取物都具有显著的抗氧化潜力,顺序如下:乙醇粗提物(CEE)>水粗提物(CAE)>己烷粗提物(CHE)。伏安法还用于评估亲水性提取物及其亚提取物的氧化还原行为。因此,结果表明存在儿茶酚类多酚,这通过色谱法和植物化学方法得到了证实。伏安分析表明是一种可靠且快速的方法,可用于确定番樱桃提取物的氧化还原行为。

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