Qi Tian-long, Zhou Yin-hui, Yuan Jing-jing, Li Zhi-song, Wang Zhong-yu, Chang Yan-zi, Zhao Er-xian, Cheng Dan, Hou Jun-kai, Tian Yan, Chen Ge-zi, Mao Yuan-yuan, Ren Yang, Zhang Li-rong, Kan Quan-cheng, Zhang Wei
Int J Clin Pharmacol Ther. 2016 Jun;54(6):462-70. doi: 10.5414/CP202547.
To determine whether ABCB1 gene polymorphisms affect the time course of action of rocuronium in Chinese patients.
This study included 105 unrelated Chinese patients undergoing general anesthesia with propofol, fentanyl, and rocuronium. Neuromuscular monitoring was performed with calibrated acceleromyography. Patients were allowed to recover spontaneously from the neuromuscular block. The time interval between the first maximum depression of the train of four (TOF) and spontaneous recovery TOF ratio of 0.25/0.7/0.8/0.9 was recorded. The Sequenom MassArray® single-nucleotide polymorphism (SNP) detection technology was used to detect the genotypes of the ABCB1 rs12720464, rs1055302. Demographic and non-genetic clinical data were also collected.
In the present study, the mean time to spontaneous recovery of TOF ratio 0.8/0.9 in ABCB1 rs12720464 GG genotype was longer compared to that observed in ABCB1 rs12720464 AG genotype (56.77 ± 14.23 minutes vs. 49.50 ± 10.49 minutes, and 62.58 ± 18.16 minutes vs. 53.20 ± 12.56 minutes, respectively, p < 0.05). Further, the time to spontaneous recovery of TOF 0.7/0.8/0.9 in ABCB1 rs1055302 GG genotype was longer than that in ABCB1 rs1055302 AG genotype (52.00 ± 12.10 minutes vs. 44.83 ± 7.38 minutes, 55.96 ± 13.92 minutes vs. 46.83 ± 7.67 minutes, 61.66 ± 17.70 minutes vs. 49.50 ± 8.44 minutes, respectively, p < 0.05).
In Chinese patients who were administered a single dose of rocuronium, the genetic variants ABCB1 rs12720464, and rs1055302 contribute to the individual< variability of time course of action.
确定ABCB1基因多态性是否影响中国患者罗库溴铵的作用时间进程。
本研究纳入105例接受丙泊酚、芬太尼和罗库溴铵全身麻醉的无血缘关系的中国患者。采用校准的加速度肌电图进行神经肌肉监测。患者从神经肌肉阻滞中自然恢复。记录四个成串刺激（TOF）首次最大抑制与自发恢复的TOF比值为0.25/0.7/0.8/0.9之间的时间间隔。使用Sequenom MassArray®单核苷酸多态性（SNP）检测技术检测ABCB1 rs12720464、rs1055302的基因型。还收集了人口统计学和非基因临床数据。
在本研究中，ABCB1 rs12720464 GG基因型患者TOF比值自发恢复至0.8/0.9的平均时间长于ABCB1 rs12720464 AG基因型患者（分别为56.77±14.23分钟对49.50±10.49分钟，以及62.58±18.16分钟对53.20±12.56分钟，p<0.05）。此外，ABCB1 rs1055302 GG基因型患者TOF自发恢复至0.7/0.8/0.9的时间长于ABCB1 rs1055302 AG基因型患者（分别为52.00±12.10分钟对44.83±7.38分钟，55.96±13.92分钟对46.83±7.67分钟，61.66±17.70分钟对49.50±8.44分钟，p<0.05）。
在单次给予罗库溴铵的中国患者中，ABCB1基因变异rs12720464和rs1055302导致了作用时间进程的个体差异。
