SPASM结构域自由基S-腺苷甲硫氨酸酶催化硫醚键形成：枯草菌素A生物合成中的Cα氢原子提取
Thioether bond formation by SPASM domain radical SAM enzymes: Cα H-atom abstraction in subtilosin A biosynthesis.
作者信息
Benjdia Alhosna, Guillot Alain, Lefranc Benjamin, Vaudry Hubert, Leprince Jérôme, Berteau Olivier
机构信息
Micalis Institute, ChemSyBio, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
INSERM U982, F-76821 Mont-Saint-Aignan, France and Institute for Research and Innovation in Biomedicine (IRIB), Regional Platform for Cell Imaging, PRIMACEN, University of Rouen, F-76821 Mont-Saint-Aignan, France.
出版信息
Chem Commun (Camb). 2016 May 7;52(37):6249-6252. doi: 10.1039/c6cc01317a. Epub 2016 Apr 18.
AlbA is a radical SAM enzyme catalyzing the formation of three unusual thioether bonds in the antibiotic subtilosin A. We demonstrate here that AlbA catalyzes direct Cα H-atom abstraction and likely contains three essential [4Fe-4S] centers. This leads us to propose novel mechanistic perspectives for thioether bond catalysis by radical SAM enzymes.
AlbA是一种自由基S-腺苷甲硫氨酸酶，可催化抗生素枯草菌素A中三个不寻常硫醚键的形成。我们在此证明，AlbA催化直接的Cα氢原子提取，并且可能包含三个必需的[4Fe-4S]中心。这使我们对自由基S-腺苷甲硫氨酸酶催化硫醚键的过程提出了新的机制观点。
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