Aktunc Erol, Oz Zehra Safi, Bektas Sibel, Altinyazar Cevdet, Koca Rafet, Bostan Serdar
School of Medicine, Department of Family Medicine, Bulent Ecevit University, 67600 Zonguldak, Turkey.
School of Medicine, Department of Medical Biology, Bulent Ecevit University, 67600 Zonguldak, Turkey.
Anal Cell Pathol (Amst). 2016;2016:6035801. doi: 10.1155/2016/6035801. Epub 2016 Mar 21.
The aim of this study was to compare the cytomorphometric characteristics of the buccal cells of Behçet's disease patients with those of healthy controls.
This case-control study compared a group of 30 patients with Behçet's disease with an age- and gender-matched control group of 30 healthy individuals. The buccal mucosal smears were stained using the Papanicolaou technique for cytomorphometric analyses. The nuclear and cytoplasmic areas were evaluated using digital image analysis; the ratio of nuclear to cytoplasmic areas and nuclear roundness are presented.
The nuclear and cytoplasmic areas of the BD patients' cells were significantly smaller than those of the healthy controls' cells, while the nucleus-to-cytoplasm ratio and neutrophil infiltration rate did not differ significantly between the groups. However, the nuclear area, cytoplasmic area, nucleus-to-cytoplasm ratio, and nuclear roundness factor were significantly higher in patients without aphthae. The neutrophil infiltration rate did not differ significantly in patients with or without aphthae.
Behçet's disease can produce cytomorphometric changes in buccal cells that are detectable by exfoliative cytology and cytomorphometric analysis techniques.
本研究旨在比较白塞病患者颊黏膜细胞与健康对照者颊黏膜细胞的细胞形态计量学特征。
本病例对照研究将30例白塞病患者与30名年龄和性别匹配的健康个体组成的对照组进行比较。颊黏膜涂片采用巴氏染色技术进行细胞形态计量分析。使用数字图像分析评估细胞核和细胞质面积；给出核质比和核圆度。
白塞病患者细胞的细胞核和细胞质面积显著小于健康对照者细胞，但两组之间的核质比和中性粒细胞浸润率无显著差异。然而，无口腔溃疡患者的细胞核面积、细胞质面积、核质比和核圆度因子显著更高。有无口腔溃疡患者的中性粒细胞浸润率无显著差异。
白塞病可导致颊黏膜细胞出现细胞形态计量学变化，这些变化可通过脱落细胞学和细胞形态计量分析技术检测到。
