Franken D R, Oehninger S, Burkman L J, Coddington C C, Kruger T F, Rosenwaks Z, Acosta A A, Hodgen G D
Department of Obstetrics and Gynecology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, Republic of South Africa.
J In Vitro Fert Embryo Transf. 1989 Feb;6(1):44-50. doi: 10.1007/BF01134581.
The hemizona assay (HZA) was developed to assess human sperm fertilizing potential. This blinded study investigated the relationship between sperm binding to the hemizona and in vitro fertilization (IVF) success (36 patients). Nonliving human oocytes were recovered from excised ovaries and stored. Each zona pellucida was cut into equal hemispheres by micromanipulation. For the HZA, one droplet exposed a hemizona to abnormal spermatozoa, while the control droplet contained the matching hemizona and spermatozoa from normal semen. After 4 hr, the number of tightly bound spermatozoa was counted. Binding to the hemizona was significantly higher for those having IVF success (mean of 36.1 +/- 7, versus 10.4 +/- 4 from the failure group; P less than 0.05). Fewer sperm from the failure group had a strictly normal morphology (3.2 versus 12.7%; P less than 0.05, Kruger method). Tight zona binding was significantly correlated with the percentage motile sperm, percentage normal morphology, and seminal sperm concentration. These results enhanced our confidence that the HZA is diagnostic for identification of patients at high risk of failing to achieve fertilization in vitro.
半透明带试验（HZA）旨在评估人类精子的受精潜力。这项盲法研究调查了精子与半透明带的结合情况与体外受精（IVF）成功率之间的关系（36例患者）。从切除的卵巢中获取非存活的人类卵母细胞并进行保存。通过显微操作将每个透明带切成相等的半球。在HZA试验中，一个液滴将一个半透明带暴露于异常精子，而对照液滴则含有匹配的半透明带和来自正常精液的精子。4小时后，对紧密结合的精子数量进行计数。IVF成功的患者精子与半透明带的结合显著更高（成功组平均为36.1±7，失败组为10.4±4；P<0.05）。失败组中形态严格正常的精子较少（3.2%对12.7%；P<0.05，克鲁格方法）。紧密的透明带结合与活动精子百分比、正常形态百分比和精液精子浓度显著相关。这些结果增强了我们的信心，即HZA可用于诊断体外受精失败风险高的患者。
