Reisinger P W, Hochstrasser K
Klinik für Hals-, Nasen- und Ohrenkranke des Klinikums Grosshadern, Universität München.
J Clin Chem Clin Biochem. 1989 Mar;27(3):169-72.
A sensitive method is described for the detection of beta 2-transferrin, a transferrin-variant found only in cerebrospinal fluid (CSF). The determination of beta 2-transferrin, whose presence is characteristic of CSF-admixtures in secretions, is performed in three steps. The proteins of the secretion are separated by polyacrylamide gel electrophoresis and then transferred electrophoretically onto a nitrocellulose sheet. Finally, the transferrins on the nitrocellulose sheet are specifically detected by an antibody reaction. The bands are visualized either by using antibodies conjugated with peroxidase or by protein A gold. With the exception of certain cases (Ritchie, R. F. & Smith, R. (1976) Clin. Chem. 22, 497-499; Görg, A. et al. (1983) Human Genetics 64, 222-226) beta 2-transferrin is found only in cerebrospinal fluid, and not in other body fluids. Therefore the detection of beta 2-transferrin can be used for the diagnosis of rhino- and otoliquorrhea. The advantage of this method is that beta 2-transferrin can be unequivocally identified by the use of a relatively small amount of technical equipment. CSF can therefore be clearly identified in secretions. An additional advantage of the method is its high sensitivity.
本文描述了一种检测β2-转铁蛋白的灵敏方法，β2-转铁蛋白是一种仅在脑脊液（CSF）中发现的转铁蛋白变体。β2-转铁蛋白的存在是分泌物中脑脊液混合的特征，其测定分三步进行。分泌物中的蛋白质通过聚丙烯酰胺凝胶电泳分离，然后通过电泳转移到硝酸纤维素膜上。最后，通过抗体反应特异性检测硝酸纤维素膜上的转铁蛋白。通过使用与过氧化物酶偶联的抗体或蛋白A金来使条带可视化。除某些情况外（Ritchie, R. F. & Smith, R. (1976) Clin. Chem. 22, 497 - 499; Görg, A.等人(1983) Human Genetics 64, 222 - 226），β2-转铁蛋白仅存在于脑脊液中，而不存在于其他体液中。因此，β2-转铁蛋白的检测可用于诊断鼻漏和耳漏。该方法的优点是使用相对少量的技术设备就能明确鉴定β2-转铁蛋白。因此，可以在分泌物中清晰地鉴定出脑脊液。该方法的另一个优点是其高灵敏度。
