在常规临床实验室环境中检测耳漏和鼻漏中的β-2转铁蛋白。
Detection of beta-2 transferrin in otorrhea and rhinorrhea in a routine clinical laboratory setting.
作者信息
Normansell D E, Stacy E K, Booker C F, Butler T Z
机构信息
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
出版信息
Clin Diagn Lab Immunol. 1994 Jan;1(1):68-70. doi: 10.1128/cdli.1.1.68-70.1994.
A simple, straightforward, and rapid method for the detection of beta-2 transferrin in otorrhea and rhinorrhea that can be used in a routine clinical laboratory is described. The beta-2 transferrin was detected by agarose gel electrophoresis of the fluid on Beckman Paragon equipment, followed by pressure transfer to a nitrocellulose membrane and then incubation with enzyme-labeled antitransferrin antibody and substrate. The procedure was fast (3.5 h) and sensitive (detected as little as 1 microgram/ml) and required only 3 microliters of fluid. Beta-2 transferrin was detected in cerebrospinal fluid diluted up to eightfold. No special training or expertise was needed, and all equipment and procedures used are commonly available in a routine clinical laboratory.
本文描述了一种简单、直接且快速的方法，用于检测耳漏和鼻漏中的β-2转铁蛋白，该方法可在常规临床实验室中使用。通过在贝克曼Paragon设备上对液体进行琼脂糖凝胶电泳来检测β-2转铁蛋白，随后进行压力转移至硝酸纤维素膜，再与酶标记的抗转铁蛋白抗体和底物孵育。该程序快速（3.5小时）且灵敏（最低可检测到1微克/毫升），仅需3微升液体。在稀释至八倍的脑脊液中也能检测到β-2转铁蛋白。无需特殊培训或专业知识，且所使用的所有设备和程序在常规临床实验室中均常见。
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