Donato-Trancoso Aline, Monte-Alto-Costa Andréa, Romana-Souza Bruna
Department of Histology and Embryology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Histology and Embryology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
J Dermatol Sci. 2016 Jul;83(1):60-9. doi: 10.1016/j.jdermsci.2016.03.012. Epub 2016 Apr 1.
The overproduction of reactive oxygen species (ROS) and exacerbated inflammatory response are the main events that impair healing of pressure ulcers. Therefore, olive oil may be a good alternative to improve the healing of these chronic lesions due to its anti-inflammatory and antioxidant properties.
This study investigated the effect of olive oil administration on wound healing of pressure ulcers in mice.
Male Swiss mice were daily treated with olive oil or water until euthanasia. One day after the beginning of treatment, two cycles of ischemia-reperfusion by external application of two magnetic plates were performed in skin to induced pressure ulcer formation.
The olive oil administration accelerated ROS and nitric oxide (NO) synthesis and reduced oxidative damage in proteins and lipids when compared to water group. The inflammatory cell infiltration, gene tumor necrosis factor-α (TNF-α) expression and protein neutrophil elastase expression were reduced by olive oil administration when compared to water group. The re-epithelialization and blood vessel number were higher in the olive oil group than in the water group. The olive oil administration accelerated protein expression of TNF-α, active transforming growth factor-β1 and vascular endothelial growth factor-A when compared to water group. The collagen deposition, myofibroblastic differentiation and wound contraction were accelerated by olive oil administration when compared to water group.
Olive oil administration improves cutaneous wound healing of pressure ulcers in mice through the acceleration of the ROS and NO synthesis, which reduces oxidative damage and inflammation and promotes dermal reconstruction and wound closure.
活性氧（ROS）的过度产生和炎症反应加剧是损害压疮愈合的主要因素。因此，由于橄榄油具有抗炎和抗氧化特性，它可能是促进这些慢性伤口愈合的良好选择。
本研究探讨了给予橄榄油对小鼠压疮伤口愈合的影响。
雄性瑞士小鼠每天接受橄榄油或水治疗，直至安乐死。治疗开始一天后，通过在皮肤上外置两块磁板进行两个周期的缺血再灌注，以诱导压疮形成。
与水组相比，给予橄榄油可加速ROS和一氧化氮（NO）的合成，并减少蛋白质和脂质的氧化损伤。与水组相比，给予橄榄油可减少炎症细胞浸润、基因肿瘤坏死因子-α（TNF-α）表达和蛋白质中性粒细胞弹性蛋白酶表达。橄榄油组的再上皮化和血管数量均高于水组。与水组相比，给予橄榄油可加速TNF-α、活性转化生长因子-β1和血管内皮生长因子-A的蛋白质表达。与水组相比，给予橄榄油可加速胶原蛋白沉积、肌成纤维细胞分化和伤口收缩。
给予橄榄油可通过加速ROS和NO的合成来促进小鼠压疮的皮肤伤口愈合，从而减少氧化损伤和炎症，促进真皮重建和伤口闭合。
