Gao Jun, Shi Yi-Ying, Zhou Hai-Feng, Ren Xu-Qin, Ji Huai
Life Science and Food Engineering School, Huaiyin Institute of Technology, Huai'an, 223001, Jiangsu, China.
Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huai'an, 223003, Jiangsu, China.
Environ Sci Pollut Res Int. 2016 Aug;23(15):15175-82. doi: 10.1007/s11356-016-6663-8. Epub 2016 Apr 20.
The effects of modified attapulgite (MA) on the dissipations of the plasticizers di-n-butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) in soil, as well as on the composition of soil microbial community, were studied. DBP, DEHP (50 mg kg(-1) in soil, respectively), and MA (1, 5, and 10 % in soil) were mixed thoroughly with soil and incubated for 60 days. DBP- and DEHP-contaminated soils without MA were used as the controls. Both of DBP and DEHP residues in bulk soils and four soil fractions were measured at five incubation times 1, 7, 15, 30, and 60 days, and their dissipation kinetic equations were analyzed. The microbial phospholipid fatty acid (PLFA) concentrations were also measured at the end of experiment. Our results showed that the effect of modified attapulgite on DBP dissipation was related to its dosage in soil. The DEHP dissipation was both inhibited by MA at the 5 and 10 % rates in soils. The application of MA changed the content percentages but did not change the concentration order of phthalate acid esters (PAEs) in soil particle-size fractions. The total microbial PLFA content was significantly increased by 5 and 10 % MA in the contaminated soils. Meanwhile, the gram-negative (GN)/gram-positive (GP) ratios increased when MA was applied at the dosages of 5 and 10 % in DBP and 10 % in DEHP-contaminated soils. Principal component analysis (PCA) indicated that the change of bacteria PLFA, especially the GN bacterial PLFA, depended on the dosages of MA added into soil. The application of MA into soil has a positive effect on reducing the eco-toxicity of PAEs in soil based on the analysis of the soil microbial PLFA.
研究了改性凹凸棒石（MA）对土壤中增塑剂邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）的消解以及对土壤微生物群落组成的影响。将DBP、DEHP（土壤中分别为50 mg kg⁻¹）和MA（土壤中分别为1%、5%和10%）与土壤充分混合并培养60天。以不添加MA的DBP和DEHP污染土壤作为对照。在培养的1、7、15、30和60天这五个时间点测定了原状土壤和四个土壤组分中DBP和DEHP的残留量，并分析了它们的消解动力学方程。在实验结束时还测定了微生物磷脂脂肪酸（PLFA）浓度。结果表明，改性凹凸棒石对DBP消解的影响与其在土壤中的用量有关。土壤中5%和10%用量的MA均抑制了DEHP的消解。MA的施用改变了土壤粒径组分中邻苯二甲酸酯（PAEs）的含量百分比，但未改变其浓度顺序。污染土壤中5%和10%用量的MA使微生物PLFA总含量显著增加。同时，在DBP污染土壤中5%和10%用量以及DEHP污染土壤中10%用量的MA施用时，革兰氏阴性（GN）/革兰氏阳性（GP）比值增加。主成分分析（PCA）表明，细菌PLFA的变化，尤其是GN细菌PLFA的变化，取决于添加到土壤中的MA用量。基于土壤微生物PLFA分析，MA施入土壤对降低土壤中PAEs的生态毒性具有积极作用。
