Kubienová Lucie, Tichá Tereza, Luhová Lenka, Petřivalský Marek
Department of Biochemistry, Faculty of Science, Palacky University, Šlechtitelů 11, 78371, Olomouc, Czech Republic.
Methods Mol Biol. 2016;1424:175-89. doi: 10.1007/978-1-4939-3600-7_15.
S-nitrosoglutathione reductase (GSNOR) is considered a key enzyme in the regulation of intracellular levels of S-nitrosoglutathione and protein S-nitrosylation. As a part of nitric oxide catabolism, GSNOR catalyzes the irreversible decomposition of GSNO to oxidized glutathione. GSNOR is involved in the regulation of plant growth and development, mediated by NO-dependent signaling mechanisms, and is known to play important roles in plant responses to various abiotic and biotic stress conditions. Here we present optimized protocols to determine GSNOR enzyme activities in plant samples by spectrophotometric measurements and by activity staining after the native polyacrylamide gel electrophoresis.
S-亚硝基谷胱甘肽还原酶（GSNOR）被认为是调节细胞内S-亚硝基谷胱甘肽水平和蛋白质S-亚硝基化的关键酶。作为一氧化氮分解代谢的一部分，GSNOR催化GSNO不可逆地分解为氧化型谷胱甘肽。GSNOR参与植物生长和发育的调节，由依赖NO的信号机制介导，并且已知在植物对各种非生物和生物胁迫条件的反应中发挥重要作用。在此，我们介绍了通过分光光度测量和天然聚丙烯酰胺凝胶电泳后的活性染色来测定植物样品中GSNOR酶活性的优化方案。
