Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 München/Neuherberg, Germany.
Free Radic Biol Med. 2018 Jul;122:110-115. doi: 10.1016/j.freeradbiomed.2017.11.027. Epub 2017 Dec 2.
Nitric oxide (.NO) acts as signaling molecule in plants being involved in diverse physiological processes such as germination, root growth, stomata closing and response to biotic and abiotic stress. S-Nitrosoglutathione (GSNO) is the storage and transport form of.NO and has a very important function in.NO signaling since it can transfer its.NO moiety to other proteins (trans-nitrosylation). The level of GSNO and thus the level of S-nitrosylated proteins are regulated by GSNO-reductase (GSNOR). In this way, this enzyme regulates the S-nitrosothiol levels and plays a balancing role in fine-tuning.NO signaling. Interestingly, oxidative post-translationally modification of GSNOR inhibited the activity of this enzyme suggesting a direct crosstalk between ROS- and RNS-signaling. In this review article the regulatory effects of ROS on GSNOR are highlighted and their physiological function in context of crosstalk between ROS and.NO and species in plants are discussed.
一氧化氮（.NO）作为植物中的信号分子，参与多种生理过程，如发芽、根生长、气孔关闭以及对生物和非生物胁迫的反应。S-亚硝基谷胱甘肽（GSNO）是.NO 的储存和运输形式，在.NO 信号转导中具有非常重要的功能，因为它可以将其.NO 部分转移到其他蛋白质上（转亚硝基化）。GSNO 的水平，因此 S-亚硝基化蛋白的水平受 GSNO 还原酶（GSNOR）的调节。通过这种方式，该酶调节 S-亚硝基硫醇的水平，并在微调.NO 信号转导中发挥平衡作用。有趣的是，GSNOR 的氧化后翻译修饰抑制了该酶的活性，表明 ROS 和 RNS 信号之间存在直接的串扰。本文重点介绍了 ROS 对 GSNOR 的调节作用，并讨论了其在 ROS 和.NO 以及植物中物种之间串扰中的生理功能。
