Eberhardt Christian, Wurnig Moritz C, Wirsching Andrea, Rossi Cristina, Rottmar Markus, Özbay Pinar S, Filli Lukas, Lesurtel Mickael, Boss Andreas
Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
Department of Visceral and Transplant Surgery, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
MAGMA. 2016 Oct;29(5):751-63. doi: 10.1007/s10334-016-0540-9. Epub 2016 Apr 19.
Diffusion-weighted magnetic resonance imaging (DW-MRI) combined with intravoxel incoherent motion (IVIM) analysis may be applied for assessment of organ lesions, diffuse parenchymal pathologies, and therapy monitoring. The aim of this study was to determine IVIM reference parameters of abdominal organs for translational research in a large cohort of C57Bl/6 laboratory mice.
Anesthetized mice (n = 29) were measured in a 4.7 T small-animal MR scanner with a diffusion-weighted echo-planar imaging sequence at the [Formula: see text]-values 0, 13, 24, 55, 107, 260, 514, 767, 1020 s/mm(2). IVIM analysis was conducted on the liver, spleen, renal medulla and cortex, pancreas, and small bowel with computation of the true tissue diffusion coefficient [Formula: see text], the perfusion fraction [Formula: see text], and the pseudodiffusion coefficient [Formula: see text]. Microvessel density (MVD) was assessed by immunohistochemistry (IHC) against panendothelial cell antigen CD31.
Mean values of the different organs [[Formula: see text] (10(-3) mm(2)/s); [Formula: see text] (%); [Formula: see text] (10(-3) mm(2)/s); MVD (MV/mm(2))]: liver 1.15 ± 0.14; 14.77 ± 6.15; 50.28 ± 33.21, 2008.48 ± 419.43, spleen 0.55 ± 0.12; 9.89 ± 5.69; 24.46 ± 17.31; n.d., renal medulla 1.50 ± 0.20; 14.63 ± 4.07; 35.50 ± 18.01; 1231.88 ± 290.61, renal cortex 1.34 ± 0.18; 10.83 ± 3.70; 16.74 ± 6.74; 810.09 ± 193.50, pancreas 1.23 ± 0.22; 20.12 ± 7.46; 29.35 ± 17.82, 591.15 ± 86.25 and small bowel 1.06 ± 0.13; 16.48 ± 3.63; 15.31 ± 7.00; 420.50 ± 168.42. Unlike [Formula: see text] and [Formula: see text], [Formula: see text] correlates significantly with MVD (r = 0.90, p = 0.037).
This systematic evaluation of murine abdominal organs with IVIM and MVD analysis allowed to establish reference parameters for future DW-MRI translational research studies on small-animal disease models.
扩散加权磁共振成像（DW-MRI）联合体素内不相干运动（IVIM）分析可用于评估器官病变、弥漫性实质病变及治疗监测。本研究旨在确定C57Bl/6实验小鼠大样本队列中腹部器官的IVIM参考参数，以用于转化研究。
对29只麻醉后的小鼠在4.7T小动物磁共振扫描仪中采用扩散加权回波平面成像序列进行测量，扩散加权值（[公式：见原文]）分别为0、13、24、55、107、260、514、767、1020 s/mm²。对肝脏、脾脏、肾髓质和皮质、胰腺及小肠进行IVIM分析，计算真实组织扩散系数（[公式：见原文]）、灌注分数（[公式：见原文]）和伪扩散系数（[公式：见原文]）。通过针对全内皮细胞抗原CD31的免疫组织化学（IHC）评估微血管密度（MVD）。
不同器官的平均值[[公式：见原文]（10⁻³ mm²/s）；[公式：见原文]（%）；[公式：见原文]（10⁻³ mm²/s）；MVD（MV/mm²）]：肝脏1.15±0.14；14.77±6.15；50.28±33.21，2008.48±419.43，脾脏0.55±0.12；9.89±5.69；24.46±17.31；未测定，肾髓质1.50±0.20；14.63±4.07；35.50±18.01；1231.88±290.61，肾皮质1.34±0.18；10.83±3.70；16.74±6.74；810.09±193.50，胰腺1.23±0.22；20.12±7.46；29.35±17.82，591.15±86.25，小肠1.06±0.13；16.48±3.63；15.31±7.00；420.50±168.42。与[公式：见原文]和[公式：见原文]不同，[公式：见原文]与MVD显著相关（r = 0.90，p = 0.037）。
通过IVIM和MVD分析对小鼠腹部器官进行的这项系统评估，能够为未来关于小动物疾病模型的DW-MRI转化研究建立参考参数。
