Maghsoodloorad Somayeh, Haghighi Ali, Sharifi Sarasiabi Khojasteh, Taghipoor Niloofar, Hosseinzadeh Nahid, Gachkar Latif, Nazemalhosseini Mojarrad Ehsan, Maghsoodloorad Elham
Dept. of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Dept. of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2016 Jan-Mar;11(1):98-103.
The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine.
One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihydropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing.
Of 118 blood samples infected by P. vivax, 46 and 72 samples belonged to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.
Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.
本研究旨在确定伊朗南部霍尔木兹甘省间日疟原虫二氢蝶酸合酶基因（dhps）的多态性，以及与间日疟原虫对磺胺多辛耐药性相关的382、383、512、553和585密码子的突变情况。
在2007 - 2008年期间制备了118份间日疟原虫分离株，用于检测二氢叶酸还原酶 - 胸苷酸合成酶（dhfr - ts）基因。这些分离株用于间日疟原虫二氢蝶酸合酶基因（dhps）的遗传多样性研究。该研究通过聚合酶链反应（PCR）检测和核苷酸测序进行。
在118份感染间日疟原虫的血样中，46份和72份样本分别来自米纳卜和贾斯克。在5个目标密码子处未检测到突变。然而，在这118份样本中，发现3株分离株（2.54%）在新的421密码子处有突变。
由于在相同样本的二氢叶酸还原酶（Pvdhfr）基因中检测到突变，但在Pvdhps基因的五个主要密码子处未发现突变，因此可以得出结论，考虑到它们在Pvdhfr中的突变，间日疟原虫在伊朗南部的霍尔木兹甘省仍对磺胺多辛敏感，因此对 Fansidar 也敏感。
