Li Yong, Guo Li-Min, Chang Ju-Pu, Xie Shou-Jiang, Piao Chun-Gen, Li Xia
Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Puyang Academy of Forestry, Puyang 457000, PR China.
Int J Syst Evol Microbiol. 2016 Jul;66(7):2617-2622. doi: 10.1099/ijsem.0.001097. Epub 2016 Apr 20.
Two Gram-staining-negative, aerobic, motile, slimy, glossy bacterial strains were isolated from bark tissue of Populus×euramericana. The bacteria grew at 10-37 °C, pH 5-10, with optimal growth at 28-30 °C, pH 6.0-8.0. Both strains grew with 0-3 % (w/v) NaCl. In the maximum-likelihood phylogenetic tree, the two isolates formed a distinct branch within the family Phyllobacteriaceae, and they were not closely related to any of the genera within the family Phyllobacteriaceae. The two novel isolates werepositive for oxidase andcatalase activity. The polar lipids profile revealed diphosphatidylglycerol, phosphatidylcholine, phospholipid, phosphatidylethanolamine, phosphatidylglycerol and five unknown lipids. The major fatty acids were C18 : 1ω7c and C16 : 0. The DNA G+C content was 56.4 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, the two strains represent a novel species belonging to a novel genus of the family Phyllobacteriaceae, for which the name Corticibacterium populi gen. nov., sp. nov. is proposed. The type strain of the type species is 16B10-2-7 (=CFCC 12884=KCTC 42249).
从欧美杨的树皮组织中分离出两株革兰氏染色阴性、需氧、具运动性、黏液状、有光泽的细菌菌株。这些细菌在10-37 °C、pH 5-10条件下生长，最适生长温度为28-30 °C，pH 6.0-8.0。两株菌株在0-3%（w/v）NaCl条件下均能生长。在最大似然系统发育树中，这两个分离株在叶杆菌科内形成一个独特的分支，且与叶杆菌科内的任何属均无密切关系。这两个新分离株的氧化酶和过氧化氢酶活性呈阳性。极性脂质谱显示有双磷脂酰甘油、磷脂酰胆碱、磷脂、磷脂酰乙醇胺、磷脂酰甘油和五种未知脂质。主要脂肪酸为C18 : 1ω7c和C16 : 0。DNA G+C含量为56.4 mol%。基于系统发育、化学分类和表型数据，这两株菌株代表叶杆菌科一个新属的一个新物种，为此提出新属名为杨树皮杆菌属（Corticibacterium），新种名为杨树皮杆菌（Corticibacterium populi）。模式种的模式菌株为16B10-2-7（=CFCC 12884=KCTC 42249）。
