Ozasa Kazunari, Won June, Song Simon, Maeda Mizuo
Bioengineering Lab., RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Department of Mechanical Convergence Engineering, Hanyang University, 222 Wangsipriro, Seongdong-gu, Seoul, 133-791, Korea.
Sci Rep. 2016 Apr 21;6:24602. doi: 10.1038/srep24602.
We demonstrate a designed interaction between two isolated cell populations of Euglena gracilis and Chlamydomonas reinhardtii, separately confined in two 25-square micro-aquariums of lab-on-chip size. The interaction was realized by interlinking two identical optical feedback systems, which measured the cell distribution. To analyze the cell populations, we measured the cell distribution in the 25 squares and irradiated the cells with a blue light pattern as an external stimulus. The cell distribution dataset was exchanged between the two systems. Governed by a designed interaction algorithm, the feedback systems produced a dynamic blue light illumination pattern that evoked the photophobic responses of both species. We also induced autonomous cell density oscillation and cell distribution separation and clustering, and analyzed how the types and diversities of the photophobic responses affected the oscillation period and separation and clustering. We conclude that artificial interlink feedback is a promising method for investigating diverse cell-cell interactions in ecological communities, and for developing soft-computing applications with living cells.
我们展示了纤细裸藻和莱茵衣藻这两个分离的细胞群体之间的一种设计好的相互作用，它们分别被限制在两个芯片实验室尺寸的25平方微米水族箱中。这种相互作用是通过将两个相同的光学反馈系统互连来实现的，这两个系统测量细胞分布。为了分析细胞群体，我们测量了25个方格中的细胞分布，并用蓝光图案作为外部刺激照射细胞。细胞分布数据集在两个系统之间交换。在一个设计好的相互作用算法的控制下，反馈系统产生了一种动态蓝光照明图案，引发了这两个物种的避光反应。我们还诱导了自主细胞密度振荡以及细胞分布的分离和聚集，并分析了避光反应的类型和多样性如何影响振荡周期以及分离和聚集。我们得出结论，人工互连反馈是一种有前途的方法，可用于研究生态群落中多样的细胞间相互作用，以及开发基于活细胞的软计算应用。
