Mavondo Greanious Alfred, Mkhwananzi Blessing Nkazimulo, Mabandla Musa Vuyisile
Discipline of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu Natal, Westville Campus, Durban, 4000, South Africa.
Malar J. 2016 Apr 21;15:226. doi: 10.1186/s12936-016-1278-6.
Malaria prevention has remained a critical area in the absence of efficacious vaccines against malaria. Drugs currently used as chemotherapeutics are also used in chemoprophylaxis increasing possible drug resistance. Asiatic acid is a natural phytochemical with oxidant, antioxidant and anti-inflammatory properties with emerging anti-malarial potential. The influence of asiatic acid administration prior to Plasmodium berghei infection of Sprague-Dawley rats on parasitaemia induction is here reported.
Sprague-Dawley rats (90-120 g) were administered with asiatic acid (10 mg/kg) 48 h before intraperitoneal infection with P. berghei. Parasitaemia induction and progression, food and water intake as well as weight were compared to 30 mg/kg chloroquine-treated and infected control rats during sub-chronic studies (21 days).
Asiatic acid pre-infection administration preserved food and water intake as well as increase in percentage weight gain of infected animals. In pre-infection treated animals, the pre-patent period was extended to day 6 from 72 h. Asiatic acid suppressed parasitaemia while oral chloroquine (30 mg/kg) did not influence malaria induction.
Per-oral, pre-infection, asiatic acid administration influenced parasitaemia patency and parasitaemia progression, food, water, and weight gain percentage. This may suggest possible chemoprophylaxis effects of asiatic acid in malaria.
在缺乏有效的疟疾疫苗的情况下，疟疾预防仍然是一个关键领域。目前用作化学治疗药物的药物也用于化学预防，这增加了产生耐药性的可能性。积雪草苷是一种天然植物化学物质，具有氧化、抗氧化和抗炎特性，具有新出现的抗疟潜力。本文报道了在斯普拉格-道利大鼠感染伯氏疟原虫之前给予积雪草苷对寄生虫血症诱导的影响。
在腹腔感染伯氏疟原虫前48小时，给斯普拉格-道利大鼠（90-120克）给予积雪草苷（10毫克/千克）。在亚慢性研究（21天）期间，将寄生虫血症的诱导和进展、食物和水的摄入量以及体重与30毫克/千克氯喹治疗并感染的对照大鼠进行比较。
感染前给予积雪草苷可保持感染动物的食物和水摄入量以及体重增加百分比。在感染前治疗的动物中，潜伏期从72小时延长至第6天。积雪草苷抑制了寄生虫血症，而口服氯喹（30毫克/千克）对疟疾诱导没有影响。
感染前口服积雪草苷影响了寄生虫血症的出现和进展、食物、水以及体重增加百分比。这可能表明积雪草苷在疟疾中可能具有化学预防作用。
