Wu Zhe, Duangmanee Promchup, Zhao Pu, Juntawong Niran, Ma Chunhong
Bioscience Department, Faculty of Science, Kasetsart University, Bangkok, Thailand; Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Plan Genetic Engineering Center of Hebei Province, Shijiazhuang, Hebei, China.
Bioscience Department, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Jundishapur J Microbiol. 2016 Jan 2;9(1):e26732. doi: 10.5812/jjm.26732. eCollection 2016 Jan.
Developing algal industries in saline-alkali areas is necessary. However, suitable strains and optimal production conditions must be studied before widespread commercial use.
The effects of light, temperature, KNO3, and CO(NH2)2 on beta-carotene and biomass accumulation were compared and evaluated in order to provide scientific guidance for commercial algal production in northeastern Thailand.
An orthogonal design was used for evaluating optimal conditions for the algal production of three candidate Dunaliella salina strains (KU XI, KU 10 and KU 31) which were isolated from saline soils and cultured in the column photobioreactor.
The optimal light and temperature for algae growth were 135.3 μmol m(-2) s(-1) and 22°C, while the conditions of 245.6 μmol m(-2) s(-1) and 22°C induced the highest level of beta-carotene production (117.99 mg L(-1)). The optimal concentrations of KNO3, CO(NH2)2, and NaHCO3 for algae growth were 0.5 g L(-1), 0.36 g L(-1), and 1.5 g L(-1), respectively, while 0, 0.12 g L(-1) and 1.5 g L(-1) were best suited for beta-carotene accumulation. The highest beta-carotene rate per cell appeared with the highest light intensity (12.21 pg) and lowest temperature (12.47 pg), and the lowest total beta-carotene content appeared at the lowest temperature (15°C). There was not a significant difference in biomass accumulation among the three Dunaliella strains; however, the beta-carotene accumulation of KU XI was higher than that of the other two strains.
Light and temperature were both relevant factors that contributed to the growth and beta-carotene accumulation of the three D. salina strains, and NaHCO3 had significantly positive effects on growth. The degree of impact of the different factors on cell growth was temperature > NaHCO3 > light intensity > KNO3 > CO (NH2)2 > strains; the impact on beta-carotene accumulation was temperature > light intensity > KNO3 > CO (NH2)2 > strains > NaHCO3.
在盐碱地发展藻类产业很有必要。然而，在广泛商业化应用之前，必须研究合适的藻种和最佳生产条件。
比较和评估光照、温度、硝酸钾和尿素对β-胡萝卜素及生物量积累的影响，为泰国东北部藻类商业化生产提供科学指导。
采用正交设计评估从盐土中分离出的三株盐生杜氏藻候选藻种（KU XI、KU 10和KU 31）在柱式光生物反应器中培养时的最佳产藻条件。
藻类生长的最佳光照和温度分别为135.3 μmol m(-2) s(-1)和22°C，而245.6 μmol m(-2) s(-1)和22°C的条件下β-胡萝卜素产量最高（117.99 mg L(-1)）。藻类生长的硝酸钾、尿素和碳酸氢钠的最佳浓度分别为0.5 g L(-1)、0.36 g L(-1)和1.5 g L(-1)，而0、0.12 g L(-1)和1.5 g L(-1)最适合β-胡萝卜素积累。单个细胞的β-胡萝卜素含量在光照强度最高（12.21 pg）和温度最低（12.47 pg）时最高，总β-胡萝卜素含量在温度最低（15°C）时最低。三株盐生杜氏藻藻种间生物量积累无显著差异；然而，KU XI的β-胡萝卜素积累高于其他两株藻种。
光照和温度都是影响三株盐生杜氏藻生长和β-胡萝卜素积累的相关因素，碳酸氢钠对生长有显著的正向影响。不同因素对细胞生长的影响程度为温度＞碳酸氢钠＞光照强度＞硝酸钾＞尿素＞藻种；对β-胡萝卜素积累的影响为温度＞光照强度＞硝酸钾＞尿素＞藻种＞碳酸氢钠。
